不同程度睡眠剥夺对大鼠认知和脑线粒体呼吸功能的影响  

The effect of sleep deprivation on cognitive and cerebral mitochondrial respiratory function in rats

作　　者：窦伟[1] 赵忠新[1] 缪明永[2] 王文昭[1] 黄流清[1] 

机构地区：[1]第二军医大学长征医院神经内科,上海200003 [2]第二军医大学生物化学与分子生物学研究室,上海200433

出　　处：《神经科学通报》2005年第3期204-209,共6页Neuroscience Bulletin

基　　金：This study was supported by grant from.the National Natural Science Foundation of China(30270487).

摘　　要：目的研究不同程度睡眠剥夺对大鼠认知和脑线粒体呼吸功能的影响。方法成年SD大鼠分为5组,分别为对照组(自由睡眠,约每天12 h),每天允许睡眠9 h组,每天允许睡眠6 h组,每天允许睡眠3 h组,全天不睡眠组即0 h组。连续10个实验日,以改良多平台睡眠剥夺法实施睡眠剥夺,以Y-型迷宫测试认知功能,断头处死后以Clark氧电极法测定脑线粒体呼吸功能。结果错误反应次数在各睡眠剥夺组均有显著增加。全天不睡眠组(0 h组)和严重睡眠限制组(3 h组)的每个研究日的错误反应次数与对照组相比,差异均有显著性(P<0.05)。睡眠剥夺各组的呼吸Ⅲ态(state 3 respiration, ST3)耗氧率有明显下降,与对照组相比差异有显著性(P<0.05)。呼吸Ⅳ态(state 4 respiration, ST4)耗氧率,只有全天不睡眠组(0h组)和对照组相比有显著降低(P<0.05)。结论不同程度的睡眠剥夺均会对认知功能及脑线粒体呼吸功能造成不利影响。Objective To investigate the effects of various degrees of sleep deprivation on cognitive and cerebral mitochondrial respiratory function in rats. Methods Sprague-Dawley rats were subjected to a 10-day sleep deprivation protocol and were divided into 5 groups randomly: allowing sleep for 12 h (control group), 9 h(group1), 6 h(group2), 3 h(group3), 0 h each day (group4). The modified multiple platform method(MMPM)was used to establish sleep deprivation model. The cognitive function was tested by Y-type maze. Mitochondrial respiratory function was measured by Clark-type oxygen electrode. Results The number of error reactions was significantly increased in sleep deprived rats. There were statistically significant differences(P<0.05)in the number of error reactions between the control group and the completely deprived(0 h group)or severely deprived(3 h group)rats on each of the study days. The oxygen consumption rate in state 3 respiration was significantly decreased(P<0.05)in sleep deprived rats compared with the control group. The oxygen consumption rate in state 4 respiration was significantly decreased(P<0.05)only in the completely deprived(0 h group)rats compared with the control group. Conclusion Sleep deprivation, no matter mild or severe, both could have adverse effects on cognitive function and cerebral mitochondrial respiratory function.

关 键 词：睡眠剥夺 大鼠 认知功能 脑线粒体 呼吸功能 神经功能 线粒体 

分 类 号：R741[医药卫生—神经病学与精神病学]