儿茶素活性成分分子印迹聚合物的分子识别特性及固相萃取研究  

Molecular Recognition Characteristics and Solid-Phase Extraction in Epigallocatechin Gallate Imprinted Polymer

作　　者：雷启福[1] 钟世安[1] 向海艳[1] 周春山[1] 于典[1] 

机构地区：[1]中南大学化学化工学院,长沙410083

出　　处：《分析化学》2005年第6期857-860,共4页Chinese Journal of Analytical Chemistry

基　　金：湖南省自然科学基金资助项目(No.04JJ3080);国家中小企业创新基金项目(No.02C26214300675)

摘　　要：以表没食子儿茶素没食子酸酯(EGCG)为模板分子,α甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,合成EGCG分子印迹聚合物。利用该聚合物对EGCG及其异构体和结构类似物进行分子识别特性研究。结果表明分子印迹聚合物对模板分子具有较高的选择性和识别能力,而空白聚合物却不具备这样的特性。在此基础上,把聚合物用于固相萃取茶叶提取物茶多酚,分离富集其中的儿茶素活性成分单体EGCG,获得了满意的效果。Molecular imprinted polymer (MIP) of epigallocatechin gallate (EGCG) was prepared by using methylacrylic (MAA) and ethylene glycol dimethacrylate ( EGDMA) as funcional and cross-linking monomers, respectively. And its molecular recognition characteristics to EGCG were evaluated by batch adsorption experiments. The results showed that the partition coefficient of EGCG bound to MIP coluld be 140.3, which was higher than that of gallocatechin-gallate(GCG), epigallocatechin (EGC) and epicatechin gallate(ECG). And high resolution factor (alpha = 2.54) between EGCG and GCG was obtained in the experiments. The EGCG-imprinted polymer exhitited high selectiveity and good recognition ability to EGCG but the blank polymer (BP) didn't. On the base of this, the possibility to selectively extract EGCG from tea polyphenols (TP) was proved by using the MIP as solid-phase extraction material.

关 键 词：分子印迹聚合物 固相萃取 活性成分 儿茶素 识别特性 Α-甲基丙烯酸 二甲基丙烯酸酯 EGCG 模板分子 没食子酸酯 结构类似物 茶叶提取物 功能单体 特性研究 分子识别 识别能力 分离富集 交联剂 乙二醇 异构体 茶多酚 

分 类 号：TQ914.1[化学工程]