用^（125）I－HOSMcAb在骨移植成骨肉瘤组织的裸鼠体上的放射免疫显像  

Radio-immuno-imaging Using Anti-humanOsteogenic Sarcoma Monnclonal AntibodyLabelled w1th ￣(125)I in Human OsteOsarcomaXenograft in tbe Bone of Nude Mice

作　　者：陈永裕[1] 陈舰[1] 宝建中[1] 徐登仁[1] 沈茜[1] 刘振华[1] 赵杰[1] 

机构地区：[1]第二军医大学附属长海医院骨科,第二军医大学病理解剖教研室,第二军医大学影像中心,第二军医大学实验诊断科

出　　处：《中华骨科杂志》1994年第11期669-671,共3页Chinese Journal of Orthopaedics

摘　　要：将人成骨肉肩细胞（ＨＯＳ－８６０３）注射入裸鼠骨髓腔，建立了裸鼠骨荷人成骨肉瘤模型，并以￣（１２５）Ｉ标记抗人成骨肉启单克隆抗体（￣（１２５）Ｉ－ＳＩＨＯＳＭｃＡｂ－Ｂ＿４），对该模型进行放射免疫显像，结果表明ＨｏＳＭｃＡｂ－Ｂ＿４对人成骨肉瘤组织的结合特异性较强，核素明显聚集于肿瘤处，而标记正常鼠ＩｇＧ对荷人成骨肉瘤模型及标记抗体对荷人肝癌模型均未见肿瘤部位显像。这些结果提。示ＨｏＳＭｃＡｂ－Ｂ＿４抗体可用于成骨肉瘤的定位显像诊断及导向治疗的载体。AbstractA xenograft of the tumour was established inthe bone of nude mice after injection of human os-teosarcoma cells ( HOS-8603)in the fembrs ofthese athymia animals。 The mice bearingxenografts showed localization of ￣(125)I-OSMcAb-B_4 determined by both gamma-camera(ECT)imaging and organ distribution studies,Radiola。belled normal immunoglobulin( IgG)shOwed notumour localization as did radiolabelled HOSM-cAb-B_4 injected into nude mice bearing xenograftof human hepatocarcinoma。These results suggestthat the potential of the HOSMcAb-B_4 antibodyfor radioimmunodetection of osteosarcomas andpossibly as a carrier for therapeutic agents.

关 键 词：肉瘤 单克隆抗体 移植瘤 放射免疫显像 骨肿瘤 

分 类 号：R738.103[医药卫生—肿瘤]