石蜡包埋B细胞性恶性淋巴瘤组织克隆性免疫球蛋白重链基因重排检测研究  

Study on Detection of Clonal Immunoglobulin Heavy Chain Gene Rearrangement in Paraffin-embedded Tissues from B-cell Lymphomas

作　　者：陈云昭[1] 李锋[1] 胡文浩[1] 李新霞[1] 李洪安[1] 蒋金芳[1] 

机构地区：[1]石河子大学医学院病理教研室/肿瘤研究中心,新疆石河子832002

出　　处：《肿瘤学杂志》2005年第3期176-178,共3页Journal of Chinese Oncology

基　　金：新疆兵团博士基金(BT2003-4);兵团医药专项(2001-841007)联合资助

摘　　要：[目的]探讨克隆性免疫球蛋白重链(IgH)基因重排在B细胞性非霍奇金淋巴瘤(B鄄NHL)诊断中的价值。[方法]采用半巢式PCR方法,对81例B鄄NHL病例,36例反应性增生及12例非淋巴瘤组织样本进行克隆性免疫球蛋白重链基因重排检测,以上病例均为经福尔马林固定的石蜡包埋组织。[结果]81例B鄄NHL中68例IgH阳性(阳性率为83.95%),36例反应性增生均为多克隆性,12例非淋巴瘤组织样本结果IgH均为阴性。[结论]克隆性免疫球蛋白重链基因重排检测可以作为诊断恶性B细胞性非霍奇金淋巴瘤的有效分子标志。Purpose] To evaluate the significance of clonal immunoglobulin heavy chain gene(IgH) rearrangement in the diagnosis for B-cell non-Hodgkin's lymphoma(B-NHL). [Methods]Semi-nested polymerase chain reaction was used to detect clonal immunoglobulin heavy chain gene rearrangement in formalin-fixed and paraffin-embedded tissues from 81 cases with B-NHL, 36 cases with reactive hyperplasia and 12 cases with non-lymphoma. [Results] Of 81 cases with B-NHL 83.95%(68/81) were positive for clonal gene immunoglobulin heavy chain gene rearrangement. All 36 cases with reactive hyperplasia were multiclonal and all non-lymphoma tissues of 12 cases were IgH negative. [Conclusion] Clonal immunoglobulin heavy chain gene rearrangement can be used as a effective diagnostic molecular marker for B-NHL.

关 键 词：淋巴瘤 非霍奇金 基因重排 B淋巴细胞 重链 基因 免疫球蛋白 

分 类 号：R733.4[医药卫生—肿瘤]