p^(16)、bcl-2基因蛋白在胃癌和癌前病变的表达及意义  

Expression and Significance of p^(16) and bcl-2 Gene in Gastric Cancer and its Precancerous Lesions

作　　者：李静[1] 李辉[2] 李舒[1] 郑学峰[1] 李潮[2] 

机构地区：[1]锦州医学院附属第一医院消化科 [2]锦州医学院附属第一医院病理科,辽宁锦州121000

出　　处：《锦州医学院学报》2005年第2期1-4,共4页Journal of Jinzhou Medical College

基　　金：辽宁省教育厅科学研究计划资助 (编号 :2 0 2 170 10 3 5 )。

摘　　要：目的　探讨p16、bcl- 2基因在胃癌发生、发展中的重要作用以及在胃癌早期诊断中的意义。方法　运用免疫组化技术检测胃癌及癌前病变中p16、bcl- 2基因蛋白的表达。结果　p16基因蛋白阳性表达率在轻、中、重度异型增生分别为6 6 . 6 7%、5 0 . 0 0 %、37 .5 0 % ,在胃癌为2 8 .5 7% ;bcl- 2基因蛋白阳性表达率在轻、中、重度异型增生分别为2 8 .5 7%、33. 33%、37 .5 0 % ,在胃癌为6 8 5 7%。p16基因蛋白的表达在轻度异型增生组及胃癌组有显著差异(P <0 . 0 0 1) ;bcl- 2基因蛋白的表达在轻度异型增生组与胃癌组(P <0 . 0 2 5 )、中度异型增生组与胃癌组(P <0 .0 1)均有显著差异。p16与bcl- 2基因蛋白表达呈显著性负相关。结论　p16基因的失活与bcl-2基因的过表达在胃癌的发生、发展中起重要作用;重度异型增生是一种重要的癌前病变;对胃异型增生粘膜进行p16、bcl- 2基因检测有助于胃癌的早期诊断和治疗。Objective To investigate the important role in the progress of carcinogenesis of stomach and the significance of p 16 and bcl-2 gene in the diagnosis of early gastric cancer. Methods Applying standard immunohistochemical S-P techniques to detecte p 16 and bcl-2 protein expression in dysplasia gastric mucosa and gastric carcinoma. Results The positive rate of p 16 in mild, moderate and severe dysplasia gastric mucosa were 66.67%, 50.00% and 37.50%; in gastric cancer it was 28.57%.The positive rate of bcl-2 in mild, moderate and severe dysplasia gastric mucosa were 33.33%, 37.50% and 62.5%; in gastric cancer it was 68.57%.The positive rates of p 16 had significant difference between mild dysplasia and gastric cancer (P <0.001).The positive rates of bcl-2 had significant difference between mild dysplasia and gastric cancer (P<0.025), moderate dysplasia and gastric cancer(P <0.05),and there was significant negative relation between the positive expression of p 16 and bcl-2 gene protein. Conclusions The inactivation of p 16 gene and over expressed of bcl-2 gene play an important role in the progress of carcinogenesis of stomach.The severe gastric dysplasia is the important precancerous lesion.Detecting the p 16 and bcl-2 gene in dysplasia gastric mucosa could predict the canceration trend and contribute to the diagnosis of gastric cancer early.

关 键 词：胃癌 癌前病变 异型增生 P^16 bcl-2 免疫组化 S^P法 

分 类 号：R735.2[医药卫生—肿瘤]