rhIL-10对脂多糖攻击小鼠血及肝IL-6和TNF-α含量的影响  

Effect of rhIL-10 on IL-6 and TNF-α levels in serum and liver of lipopolysaccharide-challenged mice

作　　者：杨红[1] 罗少洪[1] 梅寒芳[1] 李春梅[1] 

机构地区：[1]广东药学院基础学院,广东广州510224

出　　处：《中国病理生理杂志》2005年第6期1207-1209,共3页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.34051)

摘　　要：目的:研究重组人白细胞介素-10(rhIL-10)对脂多糖(LPS)诱导的血、肝IL-6和TNF-α炎症介质含量变化的影响。方法:小鼠腹腔注射LPS建立炎症模型,并同时静脉注射不同剂量的rhIL-10,ELISA法测定12h、24h、48h和72h肝组织和血清IL-6和TNF-α的含量。结果:注射rhIL-10后12h,肝组织和血清IL-6、TNF-α水平开始下调,24-48h抑制作用最明显(P<0.05),72h后抑制作用减弱,且呈剂量效应关系。结论:利用基因工程技术制备的重组人白细胞介素10(rhIL-10)显著下调肝组织和血清IL-6和TNF-α的水平。AIM: To investigate the effect of recombinant human interleukin-10 (rhIL-10) on IL-6 and TNF-α levels in serum and liver of mice exposed to lipopolysaccharide (LPS). METHODS: rhIL-10 was prepared by using genetic engineering technology. Mice were intraperitoneally with 500 μg of LPS, and then were treated intravenously with various dosages of rhIL-10. The levels of IL-6 and TNF-α in hepatic tissue and serum were determined by ELISA at 12 h, 24 h, 48 h and 72 h post rhIL-10 treatment. RESULTS: rhIL-10 markedly inhibited the increase in IL-6 and TNF-α levels in hepatic tissue and serum at 12 h after rhIL-10 treatment in LPS-challenged mice, and the inhibition effect was significant at 24-48 h after rhIL-10 treatment (P<0.05). CONCLUSION: rhIL-10 can inhibit the increase in IL-6 and TNF-α levels induced by LPS in mice.

关 键 词：白细胞介素10 白细胞介素6 肿瘤坏死因子 

分 类 号：R363[医药卫生—病理学]