层粘连蛋白对兔角膜内皮细胞bcl-2表达的影响  

Influence of laminin on the expression of bcl-2 in rabbit cornea endothelial cells

作　　者：朱晓谦[1] 吴静[1] 徐锦堂[1] 赵松滨[1] 

机构地区：[1]暨南大学医学院眼科,广东广州510632

出　　处：《中国病理生理杂志》2005年第6期1216-1219,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.39870855);广东省重点科研基金资助项目(No.2KM05203S)

摘　　要：目的:观察层粘连蛋白(LN)对兔角膜内皮细胞bcl-2表达的影响。方法:将体外培养的兔角膜内皮细胞分成3组:正常对照组、层粘连蛋白(LN)处理组、阴性对照组,采用免疫组化法和酶联免疫法分别检测3组的染色深浅及吸光度大小,以显示各组间bcl-2表达的水平。结果:免疫组织化学染色及评分结果显示LN组bcl-2为强阳性表达,正常对照组bcl-2为弱阳性表达,阴性对照组bcl-2为阴性表达。酶联免疫吸附试验检测3组吸光度分别为1.21±0.18(LN组)、1.05±0.14(正常对照组)和0.04±0.01(阴性对照组)。结论:层粘连蛋白能促进兔角膜内皮细胞bcl-2基因表达,从而可以有效地抗细胞凋亡。AIM: To investigate the influence of laminin (LN) on the expression of bcl-2 in rabbit cornea endothelial cells. METHODS: Cultured rabbit corneal endothelial cells were divided into three groups: normal control group, LN group and negative control group. Immunohistochemical technique and ELISA were used to measure the staining and A value to assess the levels of bcl-2 expression. RESULTS: Bcl-2 expression score showed that LN group had a strong positive expression. Control group only showed a weak expression. Negative control group showed a negative expression by immunohistochemical staining. The mean A value of each group were 1.21±0.18 (LN group), 1.05±0.14 (normal control group) and 0.04±0.01 (negative control group). CONCLUSION: LN promotes bcl-2 gene expression in rabbit cornea endothelial cells, and protects the cells from apoptosis.

关 键 词：层粘连蛋白 基因 BCL-2 基因表达 免 角膜 内皮细胞 

分 类 号：R363[医药卫生—病理学]