实验性精索静脉曲张诱导青春期大鼠附睾细胞凋亡的研究  

Study of experimental varicocele on cell apoptosis of epididymis in adolescent rats

作　　者：马小茹[1] 王淑秋[1] 刘月霞[2] 王淑湘[3] 陶佳南[4] 李永毅[3] 张建华[3] 付纯芳[3] 

机构地区：[1]佳木斯大学基础医学院病理生理学教研室,黑龙江佳木斯154007 [2]佳木斯大学基础医学院组胚教研室,黑龙江佳木斯154007 [3]佳木斯大学基础医学院机能实验室,黑龙江佳木斯154007 [4]佳木斯大学基础医学院中心实验室,黑龙江佳木斯154007

出　　处：《中国病理生理杂志》2005年第6期1220-1224,共5页Chinese Journal of Pathophysiology

基　　金：黑龙江省自然科学基金资助项目(No.D00-55)

摘　　要：目的:研究实验性精索静脉曲张(EVC)大鼠附睾细胞凋亡及其显微、超微结构的变化。方法:采用青春期雄性Wistar大鼠复制左精索静脉曲张模型,以末端脱氧核糖核酸转移酶介导的dUTP缺口末端标记法(TUNEL)检测细胞凋亡,常规制作附睾体部光镜、电镜标本,观察附睾组织细胞形态变化。结果:实验组(VG)细胞凋亡率显著高于假手术组(SOG)(P<0.01),实验组左、右侧细胞凋亡率有差别,但无显著意义(P>0.05)。光镜下主要改变有附睾管萎缩,上皮细胞出现空泡化,上皮内晕、亮细胞数明显增多。电镜下主要表现为主细胞内溶酶体增多、变大,残余小体增加,内质网扩张,线粒体嵴模糊,高尔基复合体空泡化;核染色质致密,形成大小不等的团块,边集于核膜处;上皮细胞游离面微绒毛稀少,可见局灶性断裂和破坏。结论:青春期大鼠实验性精索静脉曲张可致附睾组织细胞凋亡过度,使其显微及超微结构明显改变,这些变化可能是影响精索静脉曲张患者生育能力的机制之一。AIM: To study the cell apoptosis and the change of microstructure and ultrastructure in epididymis with experimental varicocele (EVC) in rats. METHODS: Experimental varicocele model was induced by partial ligation of the left renal vein in adolescent Sprague-Dawley Wistar rats. Apoptotic cells were detected by in situ terminal deoxynucleotityl transferase-mediated dTUP nick end labeling (TUNEL) technique. The corpus epididymis of the rats was prepared for light and electron microscopic observation. The microstructure and ultrastructure of the epididymis were studied. RESULTS: There was certain proportion of apoptosis cells in epididymis cells in control rats. The incidence of apoptosis increased remarkably in experimental group than that in control group (P<0.01). Under light microscope, the main changes observed were epididymis’s duct shrinked and the blebbing appeared in the epithelial cells. With electron microscope, numerous large lysosomes, increased residual bodies, expanded the endoplasmic reticulum, clouding the mitochondrion’s spinal, the vacuole in the Golgi complex, and defected main cellular organelles were also observed. Beside nuclear membrane, nuclear chromatin dense, lump, the microvilli of the columnar epithelial cells were sparse and showed local defects. CONCLUSIONS: The experimental varicocele in adolescent rats lead to an increase in cell apoptosis, microstructure and ultrastructure lesions in the epididymis, which may be another important reason of infertility resulting from varicocele.

关 键 词：精索静脉曲张 附睾 细胞凋亡 大鼠 

分 类 号：R363[医药卫生—病理学]