金雀黄素对子宫内膜癌细胞ERα和ERβmRNA水平的调节  

Regulation of genistein on the levels of ERα, ERβ mRNA in uterine endometrial cancer cells

作　　者：薛晓鸥[1] 魏丽惠[1] 

机构地区：[1]北京大学人民医院妇科,北京100044

出　　处：《北京大学学报（医学版）》2005年第3期278-280,共3页Journal of Peking University:Health Sciences

摘　　要：目的:研究金雀黄素对子宫内膜癌细胞ERα,ERβmRNA的调节作用,探讨植物雌激素对子宫内膜癌的作用机制,为临床应用植物雌激素提供理论依据。方法:体外培养子宫内膜癌细胞系Ishikawa,采用实时荧光定量RT PCR方法,观察不同剂量金雀黄素对两种雌激素亚型表达的影响,阳性对照为雌二醇、孕酮作用组。结果: (1)低剂量金雀黄素能提高子宫内膜癌细胞ERαmRNA表达,但其作用较雌二醇明显弱;低剂量金雀黄素能降低ERβmRNA的表达,而雌二醇对ERβmRNA作用不明显。(2)高剂量金雀黄素的作用与孕酮相似,能下调子宫内膜癌细胞ERαmRNA和ERβmRNA表达,但较孕酮作用弱。结论:金雀黄素对子宫内膜癌细胞ERαmRNA和ERβmRNA的表达作用不同,而且与其剂量有一定关系。推测金雀黄素有雌激素受体调节剂的作用。Objective: To explore the mechanism of phytoestrogen (genistein) on uterine endometrial cancer cells and provide the theory foundation for using phytoestrogen in clinical practice. Methods: Uterine endometrial cancer cell lines (ishikawa) were cultured in vitro at different genistein concentration, and the expressions of the two estrogen subtypes of endometrial cancer cells were observed by real-time PCR. And two treatment groups, estradiol and progestin, were used as positive controls. Results:(1) Low concentration of genistein could enhance the expression level of ERα mRNA of endometrial cancer cells, which was obviously weaker than that of the estradiol group. On the other hand, low concentration of genistein could decrease the expression level of ERβ mRNA of endometrial cancer cells, however estradiol had hardly any effect on this .(2) High concentration of genistein could decline the expression levels of ERα and ERβ mRNA of endometrial cancer cells, whose effect was weaker than that of progestin. Conclusion: The expression levels of ERα mRNA and ERβ mRNA of endometrial cancer cells vary with genistein concentrations. The experimental results suggest that genistein may act as estrogen receptor regulator.

关 键 词：金雀黄素 ERΒ MRNA水平 ERΑ 定量RT-PCR方法 雌激素受体调节剂 mRNA表达 植物雌激素 ΑMRNA 子宫内膜癌 雌二醇 调节作用 作用机制 临床应用 癌细胞系 体外培养 实时荧光 亚型表达 不同剂量 阳性对照 表达作用 低剂量 

分 类 号：R285.5[医药卫生—中药学] R322.12[医药卫生—中医学]