中国人前列腺癌VEGF-C mRNA、VEGFR-3和CD31表达与肿瘤转移的关系  

CORRELATION BETWEEN THE EXPRESSION OF VEGF-C mRNA, VEGFR-3, CD31 AND TUMOR METASTASES IN CHINESE WITH PROSTATE CANCER

作　　者：丁国芳[1] 李继承[2] 徐银峰[1] 孙瑜[1] 陶莉[1] 

机构地区：[1]浙江海洋学院医学院,舟山316004 [2]浙江大学细胞生物学研究所,杭州310031

出　　处：《实验生物学报》2005年第3期257-264,共8页Acta Biologiae Experimentalis Sinica

基　　金：浙江省教育厅科研基金(No:20040540)

摘　　要：研究前列腺癌组织VEGF-C mRNA、VEGFR-3和CD31的表达与前列腺癌中血管、淋巴管的生成及肿瘤转移的关系。取34例前列腺癌组织和12例癌周正常组织标本,应用原位杂交法检测VEGF-C mRNA表达,并经VEGFR-3和CD31免疫组织化学标记及图像分析,采用Weidner最高血管密度计数法,计数癌组织中阳性淋巴管数(MLC)和微血管密度(MVD)。前列腺癌VEGF-C mRNA阳性15例(44.12%),VEGF-C mRNA表达与前列腺癌淋巴结转移、TNM分期相关;MLC (8.26±2.73/mm^2)和MVD(74.82±11.76/mm^2)显著高于癌周正常组织的MLC(4.82±3.48/mm^2)和MVD(32.86±5.41/mm^2),两者比较具有显著性差异。VEGFR-3和CD31表达之间存在正相关。有淋巴转移和TNM分期Ⅲ、Ⅳ期的前列腺癌患者VEGF-C mRNA阳性表达和MLC、MVD分别高于无淋巴转移和Ⅰ、Ⅱ期患者,均具有显著性差异;VEGF-C mRNA表达阳性者VEGFR-3和CD31高于表达阴性者,两者比较具有显著性差异;前列腺癌在不同的组织学分级的差异无统计学意义。VEGF-C促进了肿瘤诱导的淋巴管新生和血管新生,在前列腺癌的淋巴转移中起重要作用;前列腺癌组织中的VEGFR-3和CD31表达水平与肿瘤的转移密切相关;前列腺癌组织MLC和MVD的显著增高,提示肿瘤组织有新淋巴管和血管的生成,也可作为判断肿瘤转移的生物学指标。The expressions of VEGF-C mRNA, VEGFR-3 and CD31 were studied in order to investigate the correlation between them and neoangiogenesis, hyperplasia of micro-lymphatics and tumor metastases. 34 cases of prostate cancer tissue and 12 cases of adjacent nontumorous tissue specimens were tested. They were marked by VEGFR-3 and CD31 with immunohistochemistic staining and analyzed with image, the micro-lymphatics count (MLC) and microvessel density (MVD) were counted using Weidner's highest vessel density count method; the expression of VEGF-C mRNA was inspected in situ hybridization. The expression of VEGF-C mRNA was 44.12% positive in 34 cases of prostate cancer, the MLC (8.26±2.73)mm^2 and MVD (74.82± 11.76)mm^2 in prostate cancer were significantly higher than those in adjacent nontumorous tissue (MLC, 4.82±3.48/mm^2; MVD, 32.86±5.41/mm^2). In addition, there was a correlation between the expression of VEGF-C mRNA and micro-lymphatics metastases and there was a postive correlation between the expression of VEGFR-3 and CD31. The expressions of VEGF-C mRNA, MLC, MVD in stage Ⅲ and Ⅳ and those who have lymph metastasis were higher than those in stage Ⅰ and Ⅱ and those who have no lymph metastasis; the expressions of VEGFR-3 and CD31 in VEGF-C mRNA positive groups were significantly higher than those in negative groups. The dif- ference of histopathologie grading in prostate cancer had no statistical significance. VEGF-C could accelerate the hyperplasia of micro-lymphatics and neoangiogenesis induced by tumor and play an important role in tumor lymph metastases. There was a close correlation between the expressions of VEGFR-3, CD31 and tumor metastases. The increase of MLC and MVD on prostate cancer in- dicated the hyperplasia of new micro-lymphatics and neoangiogenesis in the tumor tissue, which could also be a signal to determine the tumor metastases in clinic.

关 键 词：VEGF 前列腺癌 肿瘤转移 中国人 mRNA表达 显著性差异 免疫组织化学 癌组织 原位杂交法 微血管密度 生物学指标 淋巴管 图像分析 血管新生 TNM MLC MVD 计数法 阳性 淋巴结 正相关 性表达 统计学 分期 患者 标本 

分 类 号：R737.25[医药卫生—肿瘤]