人膀胱移行细胞癌细胞系PUMC－91的建立及其EGF受体检测  

Establishment or epithelial cell line PUMC-91 from a human bladder transitional cell carcinoma and its EGFR demonstrate

作　　者：欧阳山鹰 何祖根[1] 么崇正[1] 宋宗禄[1] 臧美孚[1] 吴德诚[1] 

机构地区：[1]北京协和医院病理科,北京协和医院外科,北京协和医院泌尿外科

出　　处：《中华泌尿外科杂志》1994年第2期104-106,共3页Chinese Journal of Urology

摘　　要：移行细胞癌细胞系ＰＵＭＣ－９１来自人膀胱乳头状移行细胞癌。材料取自７１岁反复发生膀胱乳头状移行细胞癌之女性患者，病理诊断为膀胱移行细胞癌Ⅲ级。采用电切组织原代培养后，使用１０％胎牛血清ＲＰＭＩ１６４０培养液，体外稳定传代１４０代，历时１５个月。细胞体外培养有恶性特征，裸鼠接种后有多个移植性肿瘤生长，培养细胞及移植瘤病理ＨＥ及电镜检查与原肿瘤标本相似，有移行细胞癌特点，肿瘤细胞系进行生物学特性检测，细胞倍增时间为３０ｈ，染色体分析主干系为２～４倍体。传代培养４天，核分裂指数为４．５％。乳酸脱氢酶谱检查为此细胞系所特有。培养细胞集落形成率为２５％。细胞经过￣（１２５）Ⅰ－ＥＧＦ放免受体分析表明细胞上有ＥＧＦ受体。A new human transitional cell carcinoma cell line has been established and multiplied 14 mounths through 120 passages, The PUMC 91 cells were de rived from a poorly defferentiated human urinary bladder transitional cell carcinoma,The cell line produces tumors in Balb/c nude mice after aliquots of 5×10￣6 cell/0. 2ml were inoculated subcutaneously in one mouse.PUMC-91 has been characterized cytogenitic ally and the LDH isozyme pattern was unigue. The cells can grow in soft agorse,the doubling time being 30 hours at passage 40 and 80.The morphological and ultrastructure observations demonstrated that the cells corresponded well with the malignant epithelioid morphology.The EGFRs was demonstrated on cells.

关 键 词：膀胱肿瘤 癌 组织培养 

分 类 号：R737.14[医药卫生—肿瘤]