膀胱癌P_（53）过度表达的临床意义  

The clinical significance of expression of P53 gene in human bladder cancer

作　　者：郭德荣[1] 顾方六[1] 周爱儒[1] 

机构地区：[1]北京医科大学泌尿外科研究所,北京医科大学生化教研室

出　　处：《中华泌尿外科杂志》1994年第2期113-115,共3页Chinese Journal of Urology

摘　　要：通过免疫组化ＡＢＣ法，对５０例手术切除或活检的膀胱癌标本石蜡切片进行Ｐ_（５３）基因表达的研究。结果表明：膀胱Ｐ_（５３）蛋白过度表达率为４２％（２１／５０），其中ＰＴ_２４０％（１２／３０），ＰＴ_３８２％（９／１１），ＰＴ_ａ，ＰＴ_１为０；Ｇ_２３２％（８／２５），Ｇ_３７２％（１３／１７），Ｇ_１为０。随访２５例患者，１３例Ｐ_（５３）阳性过度表达，其中有７６％（１０／１３）有复发转移，而单纯复发者只占２３％（３／１３）。１３例Ｐ_（５３）阳性表达者平均存活时间２２．６个月／例，１２例Ｐ_（５３）阴性表达者平均存活６３．７个月／例。Ｐ＜０．０１。Mutation in the gene coding for p53 tumour suppressor protein has been common in a variety of human cancer. To assess the role of a putative mutated p53 protein in human bladder cancer,a monoclonal antibody technigue was used in an immunoperoxidase detection system. A total 50 bladder cancer cases were studide 49 being transitional cell carcinomas and the remaining one squamous cell caricinoma.p53 position nuclear staining was found in 21 of 50 bladder cancer (42%),including 9 of 11 cancer with PT3, 12 of 30 cancers with PT2,13 of 17 bladder cancers with Grade 3 and 8 of 25 bladder cancers with grade 2.p53 position nulear staining was not obseved in any of 9 cancers with PTA PT1 and neither in grade 1 cancers 8.A total of 25 patients have been followuped , 10 of 13 cases having p53 position nuclear staining were found to have metasteses(76.9%).The mean survival time was compared between cases, cases with positire,p53 position nucles staining had a statistically significant poor prognostic value.(P<0. 01)

关 键 词：膀胱肿瘤 癌 免疫组织化学 

分 类 号：R737.14[医药卫生—肿瘤]