预处理对肝脏再灌注损伤大鼠肝组织、血液中NO和ET的影响及意义  

Effect of preconditioning on serum nitric oxide and endothelin in rats with ischemia-reperfusion injury

作　　者：吴亚光[1] 吴泰璜[1] 穆庆岭[1] 刘洪俊[1] 卢俊[1] 徐健[1] 苏忠学[1] 

机构地区：[1]山东省立医院,山东济南250021

出　　处：《山东医药》2003年第35期3-4,共2页Shandong Medical Journal

基　　金：山东省医药卫生科研资助项目 (No.2 0 0 1CA1DBB7)

摘　　要：目的　探讨预处理对肝脏缺血再灌注损伤大鼠肝组织和血液中一氧化氮 (NO)和内皮素 (ET)含量的影响及意义。方法　建立肝脏 70 %缺血再灌注损伤大鼠模型 ,分为对照组、缺血组、缺血预处理组、L -精氨酸组(L - arg)、Nω-硝基 - N -精氨酸甲酯 (L - NAME)组 ,观察各组肝功能变化 ,检测肝组织和血清中 NO和 ET及透明质酸 (HA)水平。结果　预处理可减轻 NO水平的下降和血浆 ET的升高 ,防止肝功酶的升高 (P<0 .0 5 )。结论　预处理可诱导缺血再灌注损伤大鼠 NO产生增加、ET产生减少 ,进而改善其微循环 ,减少再灌注损伤。Objective To evaluate the expression of nitric oxide(NO) and endothelin(ET) in rats with ischemia reperfusion injury Methods 70% hepatic ischemia reperfusion model were performed in Wister rats divided in Sham,non IPC,ischemic preconditioning(IPC 5min),L arginine,and N(G) nitro L arginine methyl esther(L NAME) group Serum and tissue NO was examied by Griess method,and serum ET and hyaluronic acid(HA) was examied by radioimmological assay Results After ischemia reperfusion,NO in serum and liver tissue was decreased and ET was increased,accopanied by microcirculation disturbance (P<0 05) Conclusion NO/ET imbalance is one cause of microcirculation disturbance after reperfusion injury

关 键 词：再灌注损伤 缺血预处理 一氧化氮 内皮素 肝脏 

分 类 号：R575.2[医药卫生—消化系统] R619[医药卫生—内科学]