免疫组化技术检测P－170糖蛋白在急性白血病患者中的表达  

DETECTION OF P-GLYCOPROTEIN EXPRESSION IN PATIENTS WIH ACUTE LEUKAEMIA AND CLINICAL SIGNIFICANCE

作　　者：冯凯[1] 周绮[1] 阎影[1] 浦津[1] 陈平[1] 楼方定[1] 

机构地区：[1]中国人民解放军总医院血液科,辽宁沈阳东北电业中心医院

出　　处：《中华内科杂志》1994年第10期666-668,共3页Chinese Journal of Internal Medicine

摘　　要：应用抗Ｐ－７０糖蛋白单克隆抗体ＪＳＢ－１及碱性磷酸酶抗碱性磷酸酶桥联免疫细胞化学染色技术对１０例正常骨髓及４２例急性白血病患者（急性淋巴细胞白血病２３例，急性非淋巴细胞白血病１９例）的骨髓细胞Ｐ－７０糖蛋白表达进行检测，以研究Ｐ－７０糖蛋白的表达与临床多药耐药之间的关系。其中初治病人Ｐ－７０糖蛋白阳性表达检出率为２８．６％（４／１４）；难治、复发病人阳性检出率为５８．８％（１０／１７）；完全缓解病人阳性检出率为９．１％（１／１１）；１０例正常骨髓标本中有１例阳性表达。１５例Ｐ－７０糖蛋白表达阳性的急性白血病患者中１２例表现为临床耐药。初步结果表明：急性白血病患者Ｐ－７０糖蛋白的表达与临床耐药性有很好的相关性，Ｐ－７０糖蛋白的检测有助于临床选择用药及判断病人预后。nti-P-glycoprotein monoclonal antibody JSB -1 and alkaline phosphatase-anti- alkaline phosphatase(APAAP)immunocytochemical staining technique were used to study the relation between P-glycopro-tein expression and clinical multidurg resistance (MDR)in 42 patients with acute leukaemia(23 ALL and 19 ANLL).10 of 17 patients who were diagnosed as refractory or relapsed acute leukaemia were positive with P-glycoprotein expression, while only 3 of 14 newly diagnosed and 1 of 11 who were in complete remission were positive. The preliminary results indicated that there was a close association between the P-glycoprotein expression and the clinical resistance to chelnotherapy in some patients.

关 键 词：白血病 单克隆抗体 P-170 糖蛋白 

分 类 号：R733.710.3[医药卫生—肿瘤]