脐带血细胞和白细胞介素-4含量与哮喘遗传表型关系的研究  

Study on genetic phenotype of asthma through examination of neonates′ cord blood cells and interleukin-4

作　　者：许以平[1] 王茂[1] 朱丽君[1] 姚苏杭[1] 邵莉[1] 葛伟国 

机构地区：[1]上海第二医科大学附属仁济医院过敏疾病防治中心,200001 [2]上海市卢湾区中心医院检验科

出　　处：《中华医学杂志》2005年第22期1547-1550,共4页National Medical Journal of China

基　　金：上海市科学技术发展基金资助项目(954119026)

摘　　要：目的通过检测哮喘产妇新生儿脐带血(以下简称脐血)来阐明与支气管哮喘发病有关的遗传表型。方法收集39例哮喘产妇分娩新生儿和46名正常产妇分娩新生儿的脐血,检测脐血中嗜酸粒细胞、嗜碱粒细胞值,总IgE、白细胞介素(IL)4含量,嗜碱粒细胞释放介质的能力;从体外培养植物血凝素(PHA)刺激的单个核细胞上清中测定IL4含量以及从纯化的嗜碱粒细胞和T淋巴细胞观察IL4mRNA的表达。结果(1)哮喘产妇组以高渗甘露醇和抗人IgE作为刺激剂,其脐血中嗜碱粒细胞的释放能力增高率分别为64.10%和15.22%,正常产妇组分别为17.95%和2.17%,两组比较P<0.005,P<0.025。(2)两组产妇嗜碱粒细胞经高渗刺激可见明显的IL4mRNA表达,但从纯化的T淋巴细胞经高渗刺激未见明显的IL4mRNA表达;从体外培养PHA刺激的从脐血分离的单个核细胞上清中所测得的IL4值两组间差异无统计学意义。(3)脐血中嗜酸粒细胞值、总IgE值、IL4值两组间差异无统计学意义。结论哮喘产妇新生儿脐血嗜碱粒细胞存在着质的异常,并明显表达IL4mRNA,提示它可能是哮喘发病有关的遗传表型。Objective To identify the genetic phenotypes of asthma based on the examination of the umbilical cord blood from neonates of the mothers with asthma. Methods Umbilical cord blood was collected from 39 asthmatic lying-in women and 46 healthy lying-in women. Eosinophils and basophils absolute counts, total immunoglobulin E, and interleukin (IL)-4 were examined. Mononuclear cells (MNCs) from the umbilical cord blood were cultured. IL-4 in the supernatant was test by ELISA. White cells were collected from the umbilical cord blood. Basophils and T lymphocytes were purified, cultured, and stimulated by hyper osmotic mannitol . RT-PCR was used to test the mRNA expression of IL-4 in the supernatant. Results There were no significant differences in the counts of basophils and eosinophils, total IgE, and IL-4 in the cord blood, and IL-4 in the supernatant of cultured MNCs. After stimulation of hyper-osmotic mannitol, equine anti-human IgE, and dust mite the HBDT positive rates were 64.10%, 17.95%, and 0% respectively in the neonates of asthmatic women group, the former two rates being both significantly higher than those in the neonates of healthy women group (15.22% and 2.17% respectively, P<0.05 and P<0.025). After stimulation of hyper-osmotic mannitol, IL-4 mRNA was markedly expressed in the purified basophils of both groups and was almost not expressed in the purified eosinophils of both groups.Conclusion There is qualitative abnormalities in the basophils from the cord blood of neonates of asthmatic mothers that strongly express IL-4 mRNA. Such characteristics may be an asthma-related genetic phenotype.

关 键 词：白细胞介素-4 脐带血细胞 表型关系 含量 IL-4MRNA 嗜碱粒细胞 mRNA表达 嗜酸粒细胞 新生儿脐带血 遗传表型 产妇分娩 细胞上清 体外培养 高渗刺激 支气管哮喘 植物血凝素 高渗甘露醇 T淋巴细胞 新生儿脐血 总IgE 释放介质 

分 类 号：R562.25[医药卫生—呼吸系统] R285.5[医药卫生—内科学]