^(125)Ⅰ-抗人成骨肉瘤单克隆抗体在荷瘤裸鼠体内的放射免疫显像  

In vivo radiolocalzation of antiosteosar-coma monoclonal antibodies (OS-McAbs) in Osteosarconia Xenografts

作　　者：徐登仁[1] 宝建中[2] 潘文舟[1] 陈永裕[3] 

机构地区：[1]第二军医大学长海医院核医学科,上海200433 [2]第二军医大学病理教研室 [3]第二军医大学长海医院骨科

出　　处：《中华实验外科杂志》1994年第1期33-34,共2页Chinese Journal of Experimental Surgery

摘　　要：利用杂交瘤技术制备了抗人成骨肉瘤单克隆抗体(OS-McAb),获得了OS-McAb_1及OS-McAb_2两株特异性抗体。经^(125)Ⅰ标记及荷瘤裸鼠体内放射免疫显像证明,OS-McAb_2明显优于OS-McAb_1,实验进一步证明OS-McAb_2对人肝细胞癌无交叉反应性。本研究为OS—McAb_2今后用于临床显像诊断及导向治疗提供了前提条件。Two antiosteosarcoma monoclonal antibodies (OS-McAbl, OS-McAb2) have been produced using hybridoma technique. Both antibodies labelled with Iodine-125 were injected in vivo into osteosarcoma xenografts, and 3 days later the xenografts could be clearly visualized by r-scintigraphy. The OS-McAb2 showed selective tumor uptake with a much higher affinity than OS-McAbl. These data indicate that OS-McAb2 may be useful for imaging and targeted therapy of human os-teogenic sarcoma.

关 键 词：成骨肉瘤 ^125I 单克隆抗体 

分 类 号：R730.3[医药卫生—肿瘤]