胰腺肿瘤DNA含量检测及生存分析  

The clinical significance of nuclear DNA content mea-surements in pancreatic neoplasms and their Kaplan-Meier survival analysis.

作　　者：孙诚谊[1] 张延龄[1] 曹世龙[1] 黄抗美[1] 李栋良[1] 蒋娉[1] 倪洪珍[1] 

机构地区：[1]上海医科大学华山医院普外科,上海医科大学肿瘤研究所放射生物实验室

出　　处：《中华外科杂志》1994年第6期364-366,共3页Chinese Journal of Surgery

摘　　要：作者对本院１９７９～１９９１年手术和病理证实的３２例胰腺癌、１０例邻近癌的胰腺组织、８例胰腺炎以及１６例胰岛细胞瘤石蜡包埋组织，用流式细胞仪（ＦＣＭ）检测其细胞核ＤＮＡ含量。结果发现：胰腺癌的异倍体率及ＤＮＡ指数明显高于胰腺非肿瘤病变者（Ｐ＜０．０１）；ＤＮＡ异倍体胰腺癌的Ｓ期比例亦明显高于二倍体胰腺癌（Ｐ＜０．０５）；胰腺癌细胞核ＤＮＡ含量的变化是一个相对独立的反映胰腺癌生物学行为的指标。ＤＮＡ二倍体胰腺癌患者的手术可切除率明显高于异倍体肿瘤患者（Ｐ＜０．０５）。采用Ｋａｐｌａｎ－Ｍｅｉｅｒ生存分析法显示，ＤＮＡ异倍体胰腺癌较二倍体者生存时间短，预后差（Ｐ＜０．０５％）。AbstractBetween 1979 and 1991, 48 cases of pancreaticparaffin-embedded specimens,ie 32 carcinomas and 16islet cell tumors, confirmed by operative and pathologi-cal examination, were studied by flow cytometric nu-clear DNA content measurements.10 specimens takenfrom the normal pancreatic tissue nearby the tumortransection margin and another 8 specimens in pancre-atitis were compared as controls. The results demon-strated that the aneuploid rate and DNA index in pan-creatic carcinoma was obviously higher than that ofnon-pancreatic tumor (P< 0.01) . In the field of pan-creatic carcinoma, the S-phase fraction was much high-er in aneuploid specimens (P<0.05) . Then the nucle-ar DNA content was a relatively independent factor toevaluate the cancerous biological behavier. The surgi-cal resectability of pancreatic tumors with diploid DNAwas significantly higher than aneuploid ones (P<0.05) , and the later carried a shorter survival timeproved by Kaplan-Meier survival analysis (P< 0.05) .Finally, the clinical significance of DNA content mea-surements in islet cell tumor was also discussed.

关 键 词：胰腺肿瘤 生存期 流式细胞仪 DNA 

分 类 号：R735.903[医药卫生—肿瘤]