抗癌基因p53在肾癌组织的异常表达及其意义  

Abnormalities of anti-oncogene p53 in renal cell carci-noma

作　　者：李鸣[1] 顾方六[1] 李万波[1] 郭德荣[1] 俞莉章[1] 周爱儒[1] 

机构地区：[1]北京医科大学泌尿外科研究所,北京医科大学生化教研室

出　　处：《中华外科杂志》1994年第8期487-489,共3页Chinese Journal of Surgery

摘　　要：研究表明，ｐ５３基因缺失或突变与众多肿瘤有关。我们采用Ｎｏｒｔｈｅｒｎｂｌｏｔ方法对肾癌组织的ｐ５３ｍＲＮＡ进行了检测。结果显示，在２１例肾癌组织中，１２例（５７％）ｐ５３ｍＲＮＡ表达异常，其中７例明显增高，５例缺失或表达明显低于正常对照。ｐ５３基因发生改变的多数是晚期和分化较差的肾癌组织，包括１例肾癌转移病灶和３例有肾静脉癌栓或肾门淋巴结转移的肾癌组织。这提示ｐ５３基因失活与肾癌密切相关，可能是肿瘤细胞向恶性发展或具有转移倾向的潜在因素之一。Abstract Mutant forms of p53 gene are found in numeroushurnan malignancies. Studies of the p53 mRNAshowed different levels of expression in manv tumours.But the relationship between the p53 gene and the re-nal cell carcinoma is less elucidated than the others. Inthis report we examined p53 gene at mRNA level byNorthern blot on the tissues from 21 patients with re-nal cell carcinoma,Abnormalities of the p53 werefound in 12(57%)tumors,in which seven had elevatedlevel of p53 mRNA,and five lacked p53 mRNA. p53gene alteration occurred in a high percentage in high-grade and advanced tumors. These results suggest thatinactivation of p53 gene is common in renal cell carci-noma and may be involved in the progression of thismalignancy.

关 键 词：肾肿瘤 癌 基因 

分 类 号：R737.110.3[医药卫生—肿瘤]