椰毒假单胞菌部分16SrRNA序列测定及其系统发育地位  

SEQUENCING OF PSEUDOMONAS COCOVENENANS PARTIAL 16S rRNA AND ITS PHYLOGENY

作　　者：曲春枫[1,2] 赵乃昕[1,2] 汪恩涛[1,2] 陈文新[1,2] 李竣[1,2] 

机构地区：[1]山东省潍坊医学院 [2]北京农业大学生物学院,中国农业科学院土壤肥料研究所

出　　处：《中华微生物学和免疫学杂志》1994年第6期387-390,共4页Chinese Journal of Microbiology and Immunology

摘　　要：采用聚合酶链反应（ＰＣＲ）扩增了椰毒假单胞菌代表菌株Ｔ７７０７的部分１６ＳｒＲＮＡ基因片段，将扩增片段克隆到Ｍ１３ｍｐ１９噬菌体，扩增后提取其单链ＤＮＡ，用ＤＮＡ测序仪进行测序。所得结果与已发表的其它已知假单胞菌的相应序列进行比较，计算出各菌之间的差异，并据此进行聚类。结果表明椰毒假单胞菌在系统发育上与Ｂｕｒｋｈｏｌｄｅｒｉａ属的成员关系密切，而与其它假单胞菌种的关系很远。这表明，椰毒假单胞菌应为Ｂｕｒｋｈｏｌｄｅｒｉａ属的一个种。Based on the study of its phenotypic characterization and DNA-DNA homology with other Pseudomonas ,the partial 16S rRNA of Pseudomonas cocovenenas was amplified by polymerase chain reaction and sequenced in DNA sequenter after the amplified gene segment was cloncd into bacteriophage M13mp19. The result was compared with those of relative bacteria that had been published. The distance was calculated and phylogenetic tree was drawn based on sequence data. With the consideration of its phenotype and the result in the research,we believe that,P. cocovenenans could belong to genus Burkholderia.

关 键 词：椰毒假单胞菌 16SrRNA 序列分析 聚合酶链反应 

分 类 号：R378[医药卫生—病原生物学]