定量分析乳腺癌细胞P^（53）基因产物的表达  

Quantitative Analysis of P￣(53) Gene Product Expression of Breast Cancer

作　　者：左连富[1] 周道安[1] 齐风英[1] 郭建文[1] 刘江惠[1] 

机构地区：[1]河北省肿瘤研究所,河北医学院病理解剖教研室

出　　处：《中华物理医学杂志》1994年第2期78-80,共3页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：应用流式免疫荧光技术，对４０例乳腺癌细胞Ｐ^（５３）基因产物表达进行了定量研究。结果表明，乳腺癌的ＤＮＡ异倍体率与组织学分级密切相关．Ｐ^（５３）产物的表达量与组织学分级相关，并且与ＤＮＡ倍体和细胞增殖活性相关。异倍体肿瘤的Ｐ^（５３）表达量明显高于二倍体肿瘤，Ｐ^（５３）表达阳性的肿瘤其ＰＩ值明显高于表达阴性的肿瘤．P￣(53) gene product expression in 40 cases of breast cancer was studied quantitatively using flow cytometry and immunofluorescence staining technique .The results showed that DNA aneuploidy rate of breast cancer was relation to histopathologic grading,and P￣(53) gene product expression content(FI value) in breast cancer was associated with pathologic grading. P￣(53) gene product expression was associated with DNA ploidy and cellular proliferation activaty.P￣(53)gene product expression content was higher in DNA aneuploid tumors than in diploid ones,PI was higher in p￣(53)expression positive tumors than in negative ones.

关 键 词：乳腺癌 P53 癌基因 DNA 流式细胞术 

分 类 号：R737.9[医药卫生—肿瘤]