宫颈粘膜癌变过程中DNA含量、Ag－NOR及核形态变化的定量分析  

Quantitative Analysis of DNA Content,Ag-NOR and Nucleus/cytoplasm Ratio during Canceration of Cervical Mucosa

作　　者：刘坤平[1] 郑杰[1] 胡文浩[1] 李洪安[1] 潘晓琳[1] 郭长军[1] 张素清[1] 高志斌[1] 吴玉新[1] 

机构地区：[1]新疆石河子医学院病理教研室,新疆石河子医学院附属医院

出　　处：《中华物理医学杂志》1994年第3期150-153,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：新疆青年医学科研基金

摘　　要：对５４例宫颈鳞状上皮不典型增生、原位癌、鳞癌Ⅰ级、Ⅱ级、Ⅲ级进行核仁组成区相关嗜银蛋白（Ａｇ－ＮＯＲ）计数研究，同时利用图像分析技术对其中的２５例进行ＤＮＡ含量的原位定量及核浆比测定。结果：随病理分级细胞ＤＮＡ含量依次增高，影响ＤＮＡ含量变化的主要因素是多倍体细胞群的出现，它的出现率是判断肿瘤恶性程度的重要指标．Ａｇ－ＮＯＲ计数及核浆比与细胞ＤＮＡ含量变化呈正相关，也随病理分级逐级增高，并在判断肿瘤恶性程度上有一定意义．The quantitative counting research of silver-binding nucleolus organizer region(Ag-NOR) was made in 54 cases of patients with cervical spuamous epithelium atypical hyperplasia,carcinoma in situ,squamous cell carcinoma grade Ⅰ,Ⅱ,Ⅲ.DNA content in situ and nucleus/cytoplasm ratio were measured with computer-assisted image analysis technique in 25 cases from 54 cases. The results showed that the DNA content increased coincidently with the pathologic grading. The appearance of hyperdiploid cell group was the main factor influencing the change on DNA content. The appearance rate was an important criterion of neoplasm malignant degree.Ag-NOR counting and nucleus/cytoplasm ratio was positively correlated with DNA content and increased coincidently with pathologic grading.This quantitave analysis is helpful for the judgement of neoplasm malignant degree.

关 键 词：宫颈癌 脱氧核糖核酸 核浆比 

分 类 号：R737.330.2[医药卫生—肿瘤]