人胎盘型谷胱甘肽S－转移酶对消化道恶性肿瘤的诊断意义  

MEASURMENT OF SERUM HUMAN PLACENTAL GLUTATHIONE S-TRANSFERASE IN DIAGNOSIS OF GASTROINTESTINAL MALIGNANCIES

作　　者：黄家淼[1] 文国义[1] 郭萍[1] 于世远[1] 赵晓宴[1] 

机构地区：[1]中国人民解放军第八一医院,第三军医大学新桥医院

出　　处：《中华消化杂志》1994年第2期92-96,共5页Chinese Journal of Digestion

摘　　要：采用谷胱甘肽亲和层析和离子交换层析，从人体胎盘提纯ＧＳＴ－π血制备兔抗ＧＳＴ－π血清，纯化其ＩｇＧ，并建立ＧＳＴ－π双抗夹心ＥＬＩＳＡ法。检测了７３例正常人和１９３例消化道良、恶性肿瘤患者血清ＧＳＴ－π含量。结果示消化道恶性肿瘤患者血清ＧＳＴ－π含量较正常人，良性肿瘤和非肿瘤患者明显增高（Ｐ＜０．０１～０．０００１）。ＧＳＴ－π对原发性肝癌、转移性肝癌、胃癌、结直肠癌和食管癌的诊断阳性率分别为９０．６％、９０．０％、６８．４％、６６．６％和５７．０％，对消化道恶性肿瘤总的诊断敏感性为８０．０％，特异性为９６．０％。Glutathione S-transferase(GST- π)Was extracted and purified from human placenta,andthe determlnation of serum GST-π by double sandwich ELISA was established.The serumGST-π concentration of healthy controls was 2.06 ± 1.41ng/ml(M±SD).Marked increase inserum GST-π was observed in patients with hepatocellular carcinoma(51.05 ± 46.51ng/m1),metastatic liver cancer(66.77 ± 56.85ng/m1),gastric cancer(16.92 ±18.03ng/ml),colorectal cancer(15.18 ±14.07ng/ml) and esophageal cancer(7,776±6.12ng/ml).These results indicate that ELISA method of determining serum GST-π is useful in serologicaldiagnosis of gastrointestihal malignancies.

关 键 词：GST ELISA 胃肿瘤 肝肿瘤 诊断 

分 类 号：R735.04[医药卫生—肿瘤]