血管活性肠肽对人胰腺癌细胞生长的自分泌调节作用及其机制的研究  

AUTOCRINE REGULATORY EFFECT OF VIP AND ITS MECHANISMS ON THE GROWTH OF A HUMAN PANCREATIC CARCINOMA CELL LINE

作　　者：陈潜[1] 郑捷[1] 盛宏森 陆国钧[1] 范振符[1] 倪莉[1] 钟守先[1] 陈元方[1] 

机构地区：[1]北京协和医院,中国科学院肿瘤医院

出　　处：《中华消化杂志》1994年第3期128-132,共5页Chinese Journal of Digestion

基　　金：国家自然科学基金

摘　　要：采用３Ｈ－胸腺嘧啶掺入研究了血管活性肠肽（ＶＩＰ）对我院所建一株人胰腺癌细胞生长的影响，并对该细胞株进行ＶＩＰ受体、受体后细胞内介质ｃＡＭＰ和多胺产生的研究。发现ＶＩＰ在１０－８Ｍ和１０－７Ｍ对具有ＶＩＰ受体的人胰腺癌细胞有明显促生长作用，且成浓度依赖关系，这种作用可被其受体拮抗剂（４－Ｃｌ－Ｄ－ｐｈｅ６，Ｌｅｕ１７）ＶＩＰ所阻断；ＶＩＰ能显著促进本株人胰腺癌细胞细胞内ｃＡＭＰ及多胺的产生，表明ＶＩＰ的促生长作用可能与细胞内ｃＡＭＰ和多胺的生物合成增加有关；本株细胞在培养过程中可向培养基中分泌一定量的ＶＩＰ，ＶＩＰ抗体能抑制其在无血清培养基中生长，提示ＶＩＰ对本株人胰腺癌细胞的生长有自分泌调节作用。In the present study,the effect of VIP on the growth of a human pancreatic carcinomacell line (PU- PAN-1)was determined by means of tritiated thymidine incorporation. VIPreceptors, intracellular cAMP and polyamines were investigated.The results indicated thatVIP at concentrations of 1O-8M to -7Mcould significantly stimulate the growth of PU- PAN- 1cells ; this effect was dose-dependent and could by abolished be VIP receptor antagonist,4-Cl- D- Phe6 ,Leu17- VIP,suggesting the mediation of VIP receptors.VIP could markedly elevate thelevel of intracellular cAMP and polyamines in PU-PAN- 1 cells,indicating the growth-prom- oting effect stimulated by VIP may be via rapid increase in biosynthesis of cAMP andpolyamines. In addition, the VIP-antibody could inhibit the growth of PU-pAN- 1 cells inserum- free culture medium.The above results suggest that this pancreatic carcinoma cell linehas autocrine regulatory effect of VIP.

关 键 词：血管活性肠肽 自分泌调节 胰腺肿瘤 

分 类 号：R735.902[医药卫生—肿瘤]