肿瘤坏死因子－α单克隆抗体阻断肝坏死发生的实验研究  

THE PROTECTIVE EFFECTS OF ANTI-MOUSE TNF MONOCLONAL ANTIBODY IN EXPERIMENTAL MASSIVE HEPATIC CELL NECROSIS MODEL

作　　者：刘沛[1] 周子文[1] 董祥家[1] 李宇权[1] 大西弘生 武藤泰敏 

机构地区：[1]沈阳市中国医科大学第二医院,日本歧阜大学第1内科

出　　处：《中华消化杂志》1994年第3期150-152,共3页Chinese Journal of Digestion

基　　金：国家自然科学基金

摘　　要：利用丙酸杆菌和内毒素引起的肝坏死模型进行了鼠肿瘤坏死因子－α（ＴＮＦ－α）单克隆抗体阻断肝坏死发生的研究。其结果：（１）鼠ＴＮＦ－α单克隆抗体有效地中和或清除了小白鼠血中的ＴＮＦ－α；（２）投与鼠ＴＮＦ－α单克隆抗体后，２４小时死亡率为１７％，较对照组（８３％）明显降低；（３）血清转氨酶明显降低，被抑制在丙酸杆菌单独投与组小白鼠血清转氨酶的水平；（４）肝组织学未发现大片肝坏死。上述结果说明ＴＮＦ－α单克隆抗体可以阻断实验性肝坏死的发生，进一步证明了ＴＮＦ－α是肝坏死发生的重要因子。We couducted an investigation on the protective effects of anti-mouse TNF monoclonalantibody m-TNF MAB)in liver cell necrosis of mice induced by injection of Pro- pionibacterium acnes and a small dose of lipopolysaccharide-endotoxin(LPS).The resultswere:1.Serum TNF was totally neutralized by the TNF MAB;2.When m-TNF MAB wasadministered 2 hrs before LPS injection,the mortality 24 hrs after the injection of LPS was17% which was significantly lower than that of control mice(83%);3.Levels of serum transaminase activity in mice treated with TNF MAB were significantaly lower than those ofcontrol mice; 4.No massive hepatic cell necrosis was found.Based on these finding,we mayconclude that anti-TNF MAB can provide some protection against massive hepatic cellnecrosis and TNF is one of the major mediators of liver injury.

关 键 词：肝坏死 肿瘤坏死因子 单克隆抗体 

分 类 号：R575.302[医药卫生—消化系统]