粗枝木麻黄(Casuarina glauca)小枝中缩合单宁提取条件的探讨  

Extraction of Condensed Tannins from Branchlets of Casuarina glauca

作　　者：黄玉杰[1] 吴彪[1] 向平[1] 林益明[1] 纪慈数[1] 向成[1] 

机构地区：[1]厦门大学生命科学学院,湿地与生态工程研究中心,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2005年第B06期32-36,共5页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金项目(B0410005)资助

摘　　要：探讨了对粗枝木麻黄(Casuarinaglauca)小枝多酚(Polyphenols)和缩合单宁(Condensedtannins)的适合提取条件.为确保样品中的多酚和缩合单宁的含量不被低估,含有1.0%HCl的7:3的丙酮水提取液是合适的提取溶剂.它能抑制以共价键结合的蛋白质和纤维素结合态的缩合单宁在样品处理过程中形成和在此酸性条件下对形成的复合物有一定的溶解,能提取出最大量的可溶态的多酚和缩合单宁.同时通过3次以上的提取,样品颗粒大小对可溶性多酚和缩合单宁的影响不显著,但是在全部过100目筛的样品研磨处理下能最多地将结合态的缩合单宁转化为可溶态.The appropriate extraction conditions of polyphenols and condensed tannins from branchlets of Casuarina glauca were discussed in this paper.Among solvent tested,the condition that contain 70% acetone and 1.0% HCl was proved to be reasonable extraction solvent.It might inhibit condensed tannins from forming complexes with proteins and fibres in plant tissues,and could redissolve the condensed tannins-protein complexes and extracted maximum amounts of polyphenols and condensed tannins in this acid solvent.In addition,the size of sample particle had no significant influence on the extraction of extractable polyphenols and condensed tannins,whereas the sample particle passing through a 100-mesh sieve can contribute to the formation of extractable condensed tannins.

关 键 词：缩合单宁 提取条件 木麻黄 小枝 共价键结合 酸性条件 处理过程 提取溶剂 水提取液 颗粒大小 可溶态 结合态 多酚 样品 复合物 纤维素 蛋白质 HCl 可溶性 

分 类 号：TS202.3[轻工技术与工程—食品科学] TQ445.3[轻工技术与工程—食品科学与工程]