基因表达调控物对视网膜母细胞瘤株动力学影响的实验研究  

Study upon the effect of gene expression regulator on the dynamics in HXO-Rb_(44) cells

作　　者：郭慧丽[1] 郭希让[1] 方风琴[1] 丁体英[1] 

机构地区：[1]河南省眼科研究所,河南郑州450003

出　　处：《河南医学研究》2005年第2期97-99,共3页Henan Medical Research

摘　　要：目的:从细胞水平上探讨基因表达调控物对视网膜母细胞瘤株动力学的影响。方法:采用基因表达调控物全反式维甲酸(ATRA)及8BrcAMP对视网膜母细胞瘤株HXORb44细胞进行体外实验,通过蛋白斑点印迹方法检测Rb44细胞繁殖细胞核抗原免疫反应(PCNAIR)的吸光度(A)值,来观察其增殖情况,流式细胞术检测细胞周期变化。结果:Rb44细胞增殖受抑制,PCNAIR的A值实验组与对照组相比,有显著差异(P<0.01)。不同时间实验组的PCNAIR的A值,与作用时间呈显著负相关(P<0.01)。且细胞周期发生改变,被阻滞于Go/G1期,S期比率下降,亦与药物作用时间有关。结论:基因表达调控物ATRA及8BrcAMP具有抑制Rb44细胞增殖作用,使其阻滞于G1期,为临床治疗视网膜母细胞瘤提供理论依据和实验基础。Objective: To clarify the effect of gene expression regulator on dynamics in HXO-Rb44 cells. Methods: The HXO-Rb44 cells in virvo were treated with all-trans retinoic acid and 8-Br-cAMP at dose of 1×10-5moI/L and 2×10-5moI/L respectively for 24 h、48 h、72 h. The proliferative cell nuclear antigen (PCNA) was detected byprotein dot blot, the cell cycle detected by flow-cytometry.Results: In AT-RA group and 8-Br-cAMP group the inhibition of Rb44 cell growth was demonstrated. The difference between experiment group and control group was significant,P<0.01,besides, there was a negative correlation between PCNA-IR A value and the days (day 1, 2, 3, P<0.01) . The effects of 8-Br-cAMP and AT-RA on the percentages of Go/G1 and S phases became increased and decreased respectively with the time lasted.Conclusion: The G1 block was induced and poliferation was inhibited by AT-RA and 8-Br-cAMP in HXO-Rb44 cells.

关 键 词：增殖 细胞周期 8-BR-CAMP 全反式维甲酸 视网膜母细胞瘤 

分 类 号：R739.7[医药卫生—肿瘤]