5aza2dc联合化疗药物诱导骨肉瘤细胞凋亡的实验研究  

Apoptosis in osteogenic sarcoma cells induced by combination of 5aza2dc and doxorubicin

作　　者：梁德勇[1] 马金萍[2] 王禹祥[1] 李建华[3] 

机构地区：[1]中国医科大学附属第一医院骨科,辽宁沈阳110001 [2]中国医科大学附属第一医院胸外科 [3]中国医科大学基础医学院病理学教研室

出　　处：《中国医科大学学报》2005年第3期249-250,共2页Journal of China Medical University

摘　　要：目的:探讨氮杂脱氧胞苷(5aza2dc)联合化疗药物对骨肉瘤疗效的影响。方法:5aza2dc、阿霉素(DOX)及两药联用处理人骨肉瘤OS732细胞24h和48h,MTT法测试细胞毒性,倒置相差显微镜、透射电镜下形态学观察。结果:0.2μmol/L的5aza2dc与1PPC的DOX联合应用24h后,肿瘤抑制率明显高于单用药组(P<0.01)。联用48h见大量细胞脱落悬浮于培养液中。电镜观察可见核固缩、核染色质边集及凋亡小体。结论:骨肉瘤OS732细胞对5aza2dc不敏感。DOX增强OS732细胞对5aza2dc的敏感性。Objective: To study the effect of the combination of 5aza2dc and doxorubicin (DOX) on the treatment of osteosarcoma. Methods: DOX, 5-aza-2′-deoxycytidine (5aza2dc),and the combination of these 2 drugs were used to treat OS-732 osteogenic sarcoma cells for 24 or 48 hours. MTT was used to evaluate the cytotoxic effects. Cellular morphologic changes were observed with phase contrast microscope and transmission electron microscope. Results: The inhibiting rate was 58.36% when 0.2 μmol/L of 5aza2dc and 1 PPC of DOX were used for 24 hours, which was much higher than that when either of the two drugs was used. Margination of condensed chromatin,karyopyknosis, and apoptotic body were observed in OS-732 cells treated with combination of 5aza2dc and DOX for 48 hours. Conclusion: Osteosarcoma OS-732 cells were not responsive to 5aza2dc-induced apoptosis. DOX enhanced the sensitivity of OS-732 cells to 5aza2dc-induced apoptosis.

关 键 词：去甲基化 阿霉素 骨肉瘤 氮杂脱氧胞苷 

分 类 号：R738.1[医药卫生—肿瘤] Q755[医药卫生—临床医学]