多形核粒细胞质膜结合酶活性的氧化损伤及IH－764－3的抗损伤作用  

OXlDATlVE DAMAGE OF PLASMA MEMBRANE-BOUNDENZYWES OF POLYMORPHONUCLEAR LEUKOCYTEAND THE EFFECT OF lH-764-3 AGAlNST DAMAGE

作　　者：贾丽[1] 法祥光[1] 卢士红[1] 刘杰文[1] 

机构地区：[1]中国医学科学院协和医科大学血液学研究所

出　　处：《中华血液学杂志》1994年第5期242-244,共3页Chinese Journal of Hematology

基　　金：国家自然科学基金;中国医学科学院青年基金

摘　　要：用乙酸肉豆寇咐醇酯（ＰＭＡ）刺激人多形核粒细胞（ＰＭＮ）可导致其质膜结合酶活性下降，碱性磷酸酶（ＡＬＰ）、５＇－书苷酸酶（５＇－ＮＴ）和中性内肽酶（ＮＥＰ）活性分别降至３６．６１％、５８．５３％和３０．７９％。及Ｈ＿２Ｏ＿２生成抑制试验证明，ＰＭＡ刺激后ＰＭＮ质膜结合酶活性的损伤乃活性氧物质（ＲＯＩ）所致。ＩＨ－７６４－３是自中药中提取的一种单体成分，经实验证明具有抗氧化损伤，保护ＰＭＮ质膜结合酶的作用，该抗损伤作用可能与其抑制Ｈ＿２Ｏ＿２生成有关。olymorphonuelear leukocytes(PMN)stimulatedwith phorbol myristate acetate (PMA) induced the de-creases of plasma membrane-bound enzyme activities ofPMN themselves.The activities of alkaline phosphatase(ALP),5'-nucleotidase (5'-NT) and neutral endopepti-dase(NEP)decreased to 36.61 %,58.53% and30. 79%,respectively.The test of and H_2O_2 pro-duction supported that the damage of the plasma mem-brane-enzymes of PMN was caused by reactive oxygenintermediates (ROI).IH-764-3, an active monomercomponent from Chinese herbs, showed the protectiveeffect to plasma membrane-bound enzymes against theoxidative damage,which was related to inhibit the gen-eration of H_2,O_2 by activated PMN themselves.

关 键 词：白细胞 质膜结合酶 氧化损伤 

分 类 号：R364.5[医药卫生—病理学]