八种细胞因子对人白血病细胞系（J6－1，J6－2）增殖的调节  

REGULATION OF CYTOKINES ON THE PROLIFERATIONOF HUMAN LEUKEMIC CELL LINES J6-1 AND J6-2

作　　者：李牧[1] 饶青[1] 应红光[1] 吴克复[1] 

机构地区：[1]中国医学科学院协和医科大学血液学研究所

出　　处：《中华血液学杂志》1994年第6期289-291,共3页Chinese Journal of Hematology

基　　金：国家自然科学基金

摘　　要：测定了Ｍ－ＣＳＦ，Ｃ－ＣＳＦ，ＧＭ－ＣＳＦ，ＩＬ－３，ＩＬ－６，ＭＧＦ，ＬＩＦ和ＴＧＦ－β＿１８种重组人生长因子单个或不同组合对白血病细胞系Ｊ６－１，Ｊ６－２集落形成率的影响。单个因子除ＴＧＦ－β＿１和ＬＩＦ表现出明显抑制效应外，其它因子均有不同程度的刺激活性。而在双因子协同下，ＴＧＦ－β＿１除与Ｍ－ＣＳＦ协同外，仍表现出明显的抑制作用，表明ＴＧＦ－β是Ｊ６－１，Ｊ６－２细胞系重要的负调节因子。从三种因子组合结果看出Ｍ－ＣＳＦ是Ｊ６－１细胞必需的细胞因子。值得注意的是，ＴＧＦ－β＿１与Ｍ－ＣＳＦ的组合对Ｊ６－１细胞有很强的增殖刺激效应。另外由Ｊ６－１细胞膜分离出的膜相关细胞因子（ＭＡＦ－Ｊ６－１）单独和与ＴＧＦ－β＿１联合，对Ｊ６－１。ffects of recombinant human cytokines including M-CSF,G-CSF,GM-CSF,IL-3,IL-6,MGF,LIF andTGF-βon colony formation of human leukemic cell linesJ6-1 and J6-2 were studied.The various stimulating ac-tivities of all cytokines on these cell lines were found ex-cept TGF-β_1·and LIF,which showed significantly in-hibitory effects. In addition,TGF-β_1 when combinedwith other cytokines except M-CSF also possessed in-hibitory activity, and ihdicated that TGF-β_1 was an im-portant negative regulation factor for J6-1 and J6-2.Thesignificant stimulating effects of M-CSF on J6-1 wasfound even when it combined with TGF-β_1.It suggestedthat M-CSF play an important role on proliferation ofJ6-1.The effects of MAF(Membrane Associated Fac-tor)-J6-1 on J6-1 and J6-2 were similar to those of M-CSF when used alone or combined with TGF-β_1.

关 键 词：重组细胞因子 白血病 细胞系 

分 类 号：R733.7[医药卫生—肿瘤]