大肠癌细胞中C－erbB－2基因扩增与临床的关系  

Amplification of C-erbB-2 oncogene in coloncarcinomas

作　　者：王亚平[1] 陈森清[1] 薛开先[1] 唐金海[1] 杨志坚[1] 

机构地区：[1]江苏省肿瘤防治研究所

出　　处：《中华医学杂志》1994年第9期536-538,共3页National Medical Journal of China

摘　　要：应用差异聚合酶链反应方法检测了２３例大肠癌组织的Ｃ－ｅｒｂＢ－２基因。结果发现在７例（３０．４３％）的大肠癌组织内存在Ｃ－ｅｒｂＢ－２基因的扩增。高中分化管状腺癌的Ｃ－ｅｒｂＢ－２扩增阳性率（３／３）高于中分化管状腺癌（４／１７）（Ｐ＜０．０５）。在已有淋巴结转移的大肠癌患者，Ｃ－ｅｒｂＢ－２扩增的阳性率为５／１３（３８．４６％），而尚无淋巴结转移患者的阳性率为２／１０（２０％），但差异无统计学意义（Ｐ＞０．２０）。ifferential PCR has been used to detect the vari-ance of c-erbB-2 gene copies in 23 colon carcinomatissues. It was found that amplification of c-erbB-2gene existed in 7 out of 23 (30%) cases. All of the threehigh-middle differentiated colon careinomas had am-plification of mrbB-2 gene in our speeimens. It washigher than that of the middle differentiated coloncarcinomas (P<0.05). The rate of amplification ofc-erbB-2 gene in patients with positive lymph nodewas approximately two times as high as that in nega-tive nodo patients, but this difference was not signifi-cant (P > 0.20).

关 键 词：基因结构 聚合酶链反应 大肠肿瘤 

分 类 号：R735.34[医药卫生—肿瘤]