准分子激光屈光性角膜切削术后兔眼角膜组织蛋白多糖的变化  

Studies on the change of proteoglycan in the corneal tissue after photorefractive keratectomy in rabbits

作　　者：赵军[1] 黄一飞[1] 陈学国[1] 巴恩平[2] 

机构地区：[1]解放军总医院眼科,北京100853 [2]解放军总医院病理科,北京100853

出　　处：《军医进修学院学报》2005年第3期170-171,共2页Academic Journal of Pla Postgraduate Medical School

基　　金："十五"军队科研基金项目(10204C)

摘　　要：目的:动态观察准分子激光屈光性角膜切削术(photorefractivekeratectomy,PRK)后角膜组织蛋白多糖(protoglycan,PG)的变化,并探讨其与角膜雾状混浊(haze)的关系。方法:16只兔(32眼)进行PRK手术建立动物模型,矫正度数-9.00D。术后裂隙灯显微镜对各组角膜的haze分级,并取不同时间点角膜组织,应用超微结构组织化学方法观察PG的变化。结果:异常PG主要沉积在角膜的前部基质;haze在术后3周明显,随着时间的推移逐渐减轻。haze愈明显,PG改变亦明显。结论:PRK术后切削区角膜基质浅层PG的异常改变与角膜haze的形成密切相关。Objective:To investive the relationship between the haze formation and the change of proteoglycan in the corneal tissue after photorefractive keratectomy(PRK).Methods:sixteen adult New Zealand White rabbits(thirty-two eyes) received PRK for –9.00D ablation. According to the time of rabbits sacrifice, they were divided into four groups, and eight eyes were in each group. They were 1 week, 3 week, 1 month and 3 month groups. After surgery, the corneas were examined periodically by slip-lamp microscopy for haze formation.the eyes were enucleated, and the corneas were studied with observation of ultrastructure histochemitry for proteoglycans. Results:The formation of haze had a tendency from mild to severe, and along with the time prolongation, it gradually decreased and disappeared. The newly synthesized abnormal proteoglycans were deposited in the anterior corneal stroma. Conclusion:The formation of haze is closely related to the deposition of proteoglycans in the anterior corneal stroma in the surgical area.

关 键 词：角膜切削术 屈光性 准分子激光 角膜混浊 角膜组织 蛋白多糖 兔 超微结构 

分 类 号：R772.2[医药卫生—眼科]