骨髓间充质干细胞分化为心肌细胞的实验研究  

Experiment study on the differentiation of mesenchymal stem cells from bone marrow into cardiomyocytes

作　　者：郭启仓[1] 李占清[1] 张宇[1] 刘志勇[1] 陈晨[1] 蒲国华[1] 

机构地区：[1]华北煤炭医学院附属医院心胸外科,唐山063000

出　　处：《中华胸心血管外科杂志》2005年第2期97-100,共4页Chinese Journal of Thoracic and Cardiovascular Surgery

摘　　要：目的　探讨骨髓间充质干细胞(MSCs)定向分化为心肌细胞的可行性,建立一种以干细胞移植为主治疗心肌梗死的治疗策略。方法　利用Percoll分离骨髓细胞培养获得MSCs;用5 氮杂胞苷(0.1、1、5、10、5 0和10 0 μmol/L)定向诱导2 4h,分别在诱导培养的第14d和2 1d ,检测细胞中的α-横纹肌肌动蛋白表达。将培养的MSCs,移植于急性梗死心肌组织内,4周后进行组织学和免疫组织化学染色,并用超声检测室壁运动和心功能改变。结果　骨髓间充质干细胞经5 氮杂胞苷诱导,培养2 1d后,5和10 μmol/L 5 氮杂胞苷诱导后的MSCs有一部分细胞表达α-横纹肌肌动蛋白,并一些细胞自发性跳动;细胞移植4周后,在缺血心肌有一部分MSCs与宿主心肌细胞形成连接并分化为具有典型的肌小节和表达α-横纹肌肌动蛋白阳性的心肌细胞。Objective To investigate the feasibility of differentiation of mesenchymal stem cells (MSCs)into cardiomyocytes. To establish a new therapeutic way for heart myocardial infarction. Methods MSCs, which were separated from bone marrow, induced by 5-azacytidine for 24 hours after being cultured and proliferated. The expression of alpha-sarcomeric actin was observed after additional 14 days and 21 days, respectively. The cultured MSCs were transplanted into the myocardial infarction site and results were observed by histological methods. Ultrasound was also used to detect the ventricular wall movement and heart function. Results The expression of alpha-sarcomeric actin was observed in part of the MSCs induced by 5-azacytidine and cultured for 21 days. Spontaneous beating of the cells was also witnessed. 4 weeks later, some MSCs that were transplanted into the acute myocardial infarction site had been differentiated into cardiomyocytes with typical sarcomeres and positive expression of alpha-sarcomeric actin. Conclusion SCs can be differentiated into cardiomyocytes.

关 键 词：干细胞分化 实验研究 骨髓间充质干细胞 横纹肌肌动蛋白 5-氮杂胞苷 免疫组织化学染色 PERCOLL 骨髓细胞培养 MSCS MSCS 干细胞移植 心功能改变 mol/L 成心肌细胞 定向分化 治疗策略 心肌梗死 定向诱导 诱导培养 蛋白表达 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]