肝癌组织中MAGE-A1表达及其临床意义  

Expression of MAGE-A1 and its clinical signficance in hepatocellular carcinoma

作　　者：肖刚[1] 张文敏[1] 张萌[1] 谢丹[1] 郭爱林[1] 文剑明[1] 

机构地区：[1]中山大学中山医学院病理学教研室,广州510080

出　　处：《临床与实验病理学杂志》2005年第3期332-334,共3页Chinese Journal of Clinical and Experimental Pathology

基　　金：广州市科技计划项目基金(2003J1C0221)

摘　　要：目的检测黑色素瘤抗原A1(MAGEA1)在肝癌组织中表达,探讨MAGEA1基因与肝细胞肝癌(HCC)患者临床病理指标的关系。方法应用RTPCR法检测31例HCC组织及相应癌旁组织中MAGEA1基因的表达。应用组织芯片和免疫组化技术分析178例HCC组织及相应癌旁组织中MAGEA1抗原表达。结果31例HCC组织标本中有21例(67.74%)表达MAGEA1基因,而相应的癌旁组织MAGEA1基因均不表达。178例HCC组织中95例表达MAGEA1抗原(53.37%),相应的癌旁组织MAGEA1抗原均不表达。结论MAGEA1基因在HCC组织中有较高表达,其阳性率与HCC患者的临床病理指标无相关关系。Purpose To investigate the expression of melanoma antigen-A1(MAGE-A1) gene in hepatocellular carcinomas and study the relationship between the expression of MAGE-A1 genes and the clinic pathological characterizations of patients with hepatocellular carcinomas (HCC). Methods The expressions of MAGE-A1 gene in 31 samples of hepatocellular carcinomas and corresponding adjacent noncancerous tissues were detected by reverse transcription-polymerase chain reaction(RT-PCR). MAGE-A1 antigen was detected using immunohistochemistry in 178 cases hepatocellular carcinomas and corresponding adjacent noncancerous tissues by tissue microarray technique. Results In the 31 hepatocellular carcinomas studied,MAGE-A1 mRNA was detected in 21 cases (67.74%). None of the corresponding adjacent noncancerous tissues expressed MAGE-A1 mRNA. In the 178 hepatocellular carcinomas studied,MAGE-A1 antigen was detected in 95 cases (53.37%). None of the corresponding adjacent noncancerous tissues expressed MAGE-A1 antigen. Conclusions MAGE-A1 gene is expressed in a high percentage of HCC,but it is unrelated to the clinic pathological characterizations of patients with HCC.

关 键 词：MAGE 肝癌组织 临床意义 表达及 临床病理指标 癌旁组织 黑色素瘤抗原 PCR法检测 免疫组化技术 HCC 肝细胞肝癌 1基因 方法应用 抗原表达 组织芯片 组织标本 相关关系 高表达 阳性率 患者 

分 类 号：R734.2[医药卫生—肿瘤] R735.7[医药卫生—临床医学]