超负荷血糖对脑缺血再灌注损伤大鼠脑细胞凋亡的研究  

Effect of Hyperglycemia on Brain Cell Apoptosis of Rats with Focal Cerebral Ischemia and Reperfusion Injury

作　　者：王洪新[1] 梅元武[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经科,430022

出　　处：《中国临床神经科学》2005年第2期161-165,共5页Chinese Journal of Clinical Neurosciences

摘　　要：目的:探讨超负荷血糖对脑缺血再灌注损伤大鼠脑细胞凋亡及凋亡相关蛋白bax、bcl 2表达的影响。方法:用Wistar大鼠腹内注射链脲佐菌素,建立超负荷糖尿病大鼠模型,之后做大脑中动脉脑缺血再灌注模型。然后对大鼠进行脑梗死体积计算并且采用免疫组织化学方法检测超负荷血糖大鼠和正常大鼠脑缺血3h再灌注2 4hbax、bcl 2的表达,同时采用TUNEL法原位标记DNA片段,检测TUNEL阳性细胞的变化,并与假手术正常对照组比较。结果:超负荷血糖组梗死面积明显大于非超负荷血糖组;同时,前者bax的表达明显高于后者而bcl 2的表达则相反;糖尿病脑缺血再灌注损伤组TUNEL阳性细胞数明显高于非糖尿病脑缺血再灌注损伤和假手术对照组。结论:超负荷血糖加重了大鼠脑缺血再灌注损伤,超负荷血糖诱发的bax、bcl 2的表达异常促进了脑细胞的凋亡,可能是其加重脑缺血再灌注损伤的机制之一。Aim:To explore the brain cell apoptotic mechanism of Wistar rats with focal cerebral ischemia reperfusion injury under hyperglycemia.Methods:Hyperglycemia model was made by injection of streptozocin through abdomen in Wistar rats; at the same time , focal cerebral ischemia injury was made by occluding the middle cerebral artery with lynon line in streptocin-induced hyperglycemic rats and then infarct volume was estimated;the bax and bcl-2 was detected through immunohistochemistry.At the same time,the terminal deoxynucleotiolyl transfer erase-mediated dUTP-biotin nick end labeling DNA fragments in situ (TUNEL) were used to detect the change of TUNEL positive cells in every group.Results:The infarct volume was higher significantly in hyperglycemic rats than which of nonhyperglycemic rats.The expression of bax enhanced greatly in hyperglycemic group compared to nonhyperglycemic group and sham-control group,the bcl-2 expression was on the contrary.The number of TUNEL positive cells in the hyperglycemic group was higher than that of nonhyperglycemic group and sham-control group . Conclusion:Hyperglycemia aggravated the injury of focal ischmia-reperfusion in Wistar rats and the enhanced expression of bax,bcl-2 may take part in brain cell apoptotic mechanism of focal ischemia reperfusion injury under hyperglycemia.

关 键 词：脑缺血再灌注损伤 超负荷血糖 脑细胞凋亡 TUNEL阳性细胞 Wistar大鼠 脑缺血再灌注模型 免疫组织化学方法 相关蛋白bax bcl-2表达 TUNEL法 链脲佐菌素 大脑中动脉 大鼠脑缺血 DNA片段 正常对照组 大鼠模型 体积计算 原位标记 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R743.302[医药卫生—临床医学]