吸烟与非吸烟肺癌病人正常肺组织中DNA加合物定量研究  

Quantitative Study on DNA-adduct in Normal Lung Tissues of Smoking and Non-smoking Lung Cancer Patlents

作　　者：张立方[1] 徐坤[1] 李学明[1] 

机构地区：[1]中国预防医学科学院环境卫生与卫生工程研究所

出　　处：《中华预防医学杂志》1994年第4期206-209,共4页Chinese Journal of Preventive Medicine

摘　　要：应用核酸酶Ｐ１介导的￣（３２）Ｐ后标记分析方法，分析了３２例胸科手术后切除的肺癌病人的正常肺组织中ＤＮＡ加合物。在３２例肺癌手术者中，２２例为吸烟者，１０例为非吸烟者。本实验吸烟组与非吸烟组肺组织ＤＮＡ加合物水平差异有显著性（Ｐ＜０．００１）；在吸烟者的放射自显影图片上出现一条斜的放射带。相关性分析表明，随着吸烟量的增加，肺组织中ＤＮＡ加合物也增加，有显著相关（ｒ＝０．７３，Ｙ＝０．４１１Ｘ＋３．５２，Ｐ＜０．０１）。ung tissues from 32 cases of lung cancer with surgical operation, 22 smokers and 1 nonsmokers.were analyzed with Pl-mediated modification of￣(32)P-postlabelling teehnique. The ievel of lung DNA-adduct in cigarette smokers was higher than that in nonsmokers with significant difference(P<0.001).Autoradiogram showed a diagonal radiOactive zone in smokers' lung tissues.Correlation analysis indicated DNA-adduct in lung tissues increased with the number of cigarettes smoked with a regression equation of Y=0.411X+3.52,r=0.74, P<0001.

关 键 词：吸烟 肺肿瘤 病因 

分 类 号：R734.202[医药卫生—肿瘤]