^(99)Tc^m-DTPA-DG的制备及其荷瘤裸鼠实验研究  

Preparation and experimental study of ^(99)Tc^m-DTPA-deoxyglucose for tumor imaging

作　　者：陈跃[1] 黄占文[1] 何菱[2] 郑时龙[2] 李举联[2] 秦大莲[3] 

机构地区：[1]泸州医学院附属医院核医学科,646000 [2]四川大学华西药学院 [3]泸州医学院药学院

出　　处：《中华核医学杂志》2005年第3期176-178,共3页Chinese Journal of Nuclear Medicine

基　　金：四川省教育厅自然科学研究基金资助项目(2004B005)

摘　　要：目的进行99Tcm-DTPA脱氧葡萄糖(DG)的化学合成、标记物制备、质量控制及药理研究。方法合成的DTPADG用氯化亚锡作还原剂,与99TcmO-4混合,在25℃以上室温放置30min或沸水浴反应10min,用一步法进行99Tcm标记。丙酮和质量分数0.9%生理盐水作展开剂,用纸层析法鉴定99Tcm-DTPADG的放化纯、标记稳定性;进行乳腺癌MCF7裸鼠体内生物分布实验及显像研究。结果标记产物放化纯>99%。99Tcm-DTPADG荷瘤裸鼠生物分布显示,肿瘤血液比值1、2h分别为1.29、3.13,肿瘤肌肉比值1、2h分别为2.63、5.01。荷瘤裸鼠99Tcm-DTPADG显像显示肿瘤组织。结论99Tcm-DTPADG可能成为肿瘤葡萄糖代谢显像剂。Objective To study the chemical synthesis, preparation, quality control and pharmacological properties of 99Tcm-DTPA-deoxyglucose (DG). Methods DTPA-DG was synthesized and labeled with 99Tcm by stannous chloride reduction method. Paper chromatography was performed with acetone and physiological saline as the mobile phase. The biodistribution and scintigraphic imaging after 99Tcm-DTPA-DG administration in MCF-7 breast carcinoma cell bearing nude mice were fulfilled. Results A kit developed with the synthetic DTPA-DG and stannous chloride. DTPA-DG was labeled by adding 99Tcm, stand 30 min at a room temperature or boiled 10 min. High radiochemical purity reached 99%. High tumor/blood ratios (1.29, 3.13 at 1, 2 h) and tumor/muscle ratios (2.63, 5.01 at 1, 2 h) were observed. Scintigraphic results demonstrated the concentration of 99Tcm-DTPA-DG in tumor site. Conclusion 99Tcm-DTPA-DG may be used as a potential tumor imaging agent.

关 键 词：^99TC^M-DTPA 荷瘤裸鼠 实验研究 ^99Tc^mO4^- 制备 ^99TC^M标记 0.9%生理盐水 体内生物分布 脱氧葡萄糖 MCF-7 葡萄糖代谢 化学合成 药理研究 质量控制 氯化亚锡 质量分数 纸层析法 显像研究 肿瘤组织 标记物 还原剂 

分 类 号：R73-36[医药卫生—肿瘤]