雌激素受体α、β亚型在子宫平滑肌瘤中的表达特征  

Characteristics of expression of estrogen receptor-α and -β in uterine leiomyomas

作　　者：张桂香[1] 赵学东[2] 王文勇[3] 郑纪宁[3] 梅立新[3] 许倩[3] 张金环[1] 孙春荣[4] 

机构地区：[1]承德医学院附属医院妇产科,承德067000 [2]承德医学院附属医院放射科,承德067000 [3]承德医学院附属医院生化病理教研室,承德067000 [4]河北省人民医院妇产科

出　　处：《解剖学杂志》2005年第3期284-286,i001,共4页Chinese Journal of Anatomy

摘　　要：目的:探讨雌激素受体(ER)α、β亚型的表达量在子宫平滑肌瘤发病中的意义。方法:取自愿手术的子宫肌瘤患者30例为研究对象,应用非放射性原位杂交技术方法测定平滑肌瘤组织中ERα和ERβmRNA的表达,并与正常的平滑肌组织相比较。结果:ERαmRNA和ERβmRNA主要在平滑肌细胞中表达。子宫肌瘤和正常的平滑肌组织中ERαmRNA的表达量高于ERβmRNA;ERαmRNA在子宫肌瘤中的表达明显高于正常的平滑肌组织,而ERβmRNA的表达仅呈下降趋势。结论:子宫肌瘤的发生发展可能与ERαmRNA的高表达有关,亦可能与ERβmRNA减少趋势有关。Objective: To study the expression of estrogen receptor (ER)-α and -β messenger RNA(mRNA) in uterine leiomyomas. Methods: Samples of uterine leiomyomas and myometriums were obtained from the patients during surgery. Expression of ER-α and ER-β mRNA was detected with nonradioactive in situ hybridization (ISH) techniques. Results: ER-α and ER-β mRNA were mainly localized in uterine smooth muscle cells, but ER-α was significantly higher than ER-β. Expression of ER-α mRNA in leiomyomas was higher than that in myometrium. The expression of ER-β showed a decreasing trend in leiomyoma. Conclusion: The increase of ER_ α mRNA expression and decrease of ER_ β mRNA expression are related to the development of leiomyoma. The imbalance in expression of ER subtypes contributes to the pathogenesis of leiomyoma.

关 键 词：子宫平滑肌瘤 雌激素受体Α Β亚型 表达特征 ΑMRNA 平滑肌组织 放射性原位杂交 子宫肌瘤 ERβ 平滑肌细胞 研究对象 肌瘤组织 技术方法 下降趋势 发生发展 表达量 高表达 

分 类 号：R737.33[医药卫生—肿瘤] R737.9[医药卫生—临床医学]