重组人白细胞介素-11对放射后小鼠肠道粘膜免疫系统的保护作用的实验研究  

Protection of intestinal mucosal immune function by recombinant human interleukin-11 after radiation injury in mice

作　　者：钟波[1] 徐辉[1] 粟永萍[1] 冉新泽[1] 王连友[1] 

机构地区：[1]第三军医大学预防医学院防原医学教研室,全军复合伤研究所,烧伤,创伤与复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2005年第12期1256-1258,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助重点资助项目 ( 30 2 30 36 0 ) ;国家自然科学基金资助面上项目 ( 30 2 70 4 2 2 )~~

摘　　要：目的　探讨重组人白细胞介素 11(rhIL 11)对放射后肠道粘膜免疫功能的保护作用。方法　采用6 0 Coγ射线12Gy全身电离辐射致轻度肠型放射病的小鼠动物模型,用PP、IEL计数,免疫酶联吸附、细菌培养、血浆内毒素测定等方法,观察rhIL 11对肠道粘膜免疫系统的保护作用。结果　rhIL 11能增加全肠PP结,IEL数量,提高肠粘液中sIgA的含量,降低肠源性感染的发生,降低血浆内毒素的含量。结论　rhIL 11对放射后肠道粘膜免疫系统具有一定的保护作用。Objective To investigate the protective effects of recombinant human interleukin-11 (rhIL-11) on immune function of intestinal mucosa after radiation in mice. Methods Eighty male BALB/c mice were divided into normal group, irradiation group and rhIL-11 treatment group. Except the normal group, the mice were irradiated with 60 Co γ-ray of 12 Gy. The rhIL-11 treatment group was subcutaneously injected 125 μg/kg rhIL-11 twice a day till death. PP knot in entire intestine and IEL in intestinal mucosa and the colony in bacterial cultures were counted. The sIgA in intestinal mucosa and serum endotoxin were detected. Results rhIL-11 increased sIgA, PP, IEL, and reduced the incidence of enterogenous infection. Conclusion rhIL-11 has protective effects on the immune function of intestinal mucosa after radiation injury in mice.

关 键 词：RHIL-11 电离辐射 肠道 粘膜免疫 

分 类 号：R322.45[医药卫生—人体解剖和组织胚胎学] R392.11[医药卫生—基础医学]