放射标记抗HBx单克隆抗体在荷人肝癌裸鼠模型中的放射免疫定位研究  

RADIOIMMUNOIMAGING WITH MONOCLONAL ANTIBODY TO HBV A PROTEIN IN NUDE MICE BEARING HUMAN HEPATOCELLULAR CARCINOMA

作　　者：李君[1] 汤钊猷[1] 刘康达[1] 陈绍亮 徐开平 CH Schroder 

机构地区：[1]上海医科大学肝癌研究所 [2]中山医院核医学科 [3]德国癌症研究中心

出　　处：《中华肿瘤杂志》1994年第1期11-14,共4页Chinese Journal of Oncology

摘　　要：乙型肝炎病毒Ｘ抗原同原发性肝细胞癌之间的密切关系已引起人们的广泛注意。研究表明ｘ抗原在肝癌组织中的表达活跃，抗ＨＢｘ抗体对肝癌组织有较强的特异性亲和力。本实验同德国癌症中心合作，应用基因工程技术生产的Ｘ抗原免疫动物，成功地制备了抗ＨＢｘ单克隆抗体，并应用１３１Ⅰ标记抗ＨＢｘ单抗进行了人肝癌裸鼠模型的放射免疫显像及放射组织分布研究。γ照相结果显示实验组给药后第３天肿瘤即获阳性显像，第７天时更加清晰，而对照组未获阳性显像，放射性物质呈弥散分布。实验组瘤／血比值由第１天０．４８增至第７天的１．５，瘤／肝比值由１．２增至４．５，与对照组相比差异均有显著性意义。结果提示抗ＨＢｘ单抗与肝癌组织有较强的特异性亲和力，为抗ＨＢｘ单抗作为肝癌导向治疗的载体提供了依据。Abstract A mouse monoclonal antibody directed against the protein of hepatitis B virus X open readingframe was prepared. After radiolabeled with sodium131Ⅰ-iodine and administration,imaging wasperformed with gamma camera and radioactivity of the rumor,organs,and blood were counted in amini-gamma counter at day 1,3,5,7 in nude mice bearing HCC xenografts,and the biodistribution andT:NT ratio was calculated. Selective accumulation was observed in tumor sites on day 3 and a clearimage of tumor was shown in gamma camera on day 7. The image of tumor in the irrelevant IgGgroup was not obtained at any time.The tumor:blood ratio was 0.48 and tumor:liver was 1.2 onday 1,then increased to 1.5 and 4.5 on day 7 in experimental group,respectively. Specific mAbuptake by tumor was significantly greater than nonspecific IgG(P<0.05) on day 7.The results wereencouragin.The finding of this study indicates the possibility of using 131Ⅰ-anti-HBx mAb totarget cytostatic drugs to HCC.

关 键 词：肝肿瘤 单克隆抗体 放射免疫测定 

分 类 号：R735.7[医药卫生—肿瘤]