CD_3单克隆抗体活化的杀伤细胞对人胃癌细胞株MNK_（45）体外杀伤及裸鼠体  

EXPERIMENTAL STUDY OF THE ANTI-TUMOR ACTIVITY OF CD3AK AGAINST HUMAN GASTRIC CANCER CELL LINE IN VITRO AND IN VIVO

作　　者：白云飞[1] 张学庸[1] 牟震先[1] 吴觉平[1] 

机构地区：[1]西安第四军医大学西京医院消化内科,北京中国人民解放军第二六三医院内一科

出　　处：《中华肿瘤杂志》1994年第2期111-114,共4页Chinese Journal of Oncology

摘　　要：用人的外周血单个核细胞（ＰＢＭＣ）与抗ＣＤ３单克隆抗体和基因重组的人ＩＬ－２，共同培养制备ＣＤ３ＡＫ细胞（ＣＤ３ＭｃＡｂＡｃｔｉｖａｔｅｄＫｉｌｌｅｒＣｅｌｌｓ），ＰＢＭＣ与ｒＩＬ－２共同培养制备ＬＡＫ细胞，用单纯ＰＢＭＣ作为对照细胞。对这三种细胞的增殖及在体外和裸鼠体内的抗人胃癌细胞株ＭＮＫ４５作用进行了实验研究。体外试验结果表明，ＣＤ３ＡＫ对ＭＮＫ４５有明显杀伤作用，而ＬＡＫ细胞杀伤率较低，二者差异有非常显著性意义（Ｐ＜０．０１）；用的ＭＴＴ法和台盼蓝活细胞计数法测定这三种细胞的增殖能力和细胞数量的增加，表明ＣＤ３ＡＫ的增殖能力和扩增数量均明显高于ＬＡＫ细胞（Ｐ＜０．０１）。裸鼠体内试验结果显示，对照组及ＬＡＫ组全部长出瘤结节，而ＣＤ３ＡＫ组无一例长出皮下结节，且生存期明显长于前者。Abstract Human CD3AK and LAK cells were prepared from peripheral blood mononuclear cells(PBMC)by culturing them in recombinant IL-2(rIL-2,30 u/ml)and anti-CD3 monoclonalantibody,and in rIL-2 alone(300/ml),respectively.By MTT assay,it was found that the PBMC,when cultured in the presence of anti-CD3/rIL-2,proliferated more actively and persistenlythan PBMC cultured in the presence of rIL-2 alone. In vitro cytotoxicity assay showed that CD3AKcells had stronger killing activity against a poorly differentiated human gastric adenocarcinoma cell line MNK 45 than LAK cells did. Winn's assay at an E/T ratio of 20 carried out in nude mice alsoindicated that CD3AK cells were more effective than lAK cells in tumor growth inhibition.When thenude mice were inoculated with MNK 45 admixed with CD3AK,none of them developed tumorwhereas those inoculated with either MNK45 or MNK 45 admixed with LAK cells all developed tumor,The results indicate that CD3AK would be a better choice than LAK for the adoptive immumotherapy of human stomach cancer.

关 键 词：胃肿瘤 单克隆抗体 杀伤细胞 

分 类 号：R735.2[医药卫生—肿瘤]