特种纤维素酶催化水解壳聚糖及壳寡糖的制备研究  

Study on the Chitosan Hydrolysis Catalyzed by Special Cellulase and Preparation of Chitooligosaccharide

作　　者：刘羿君[1] 蒋英[2] 封云芳[1] 韩冬林[3] 

机构地区：[1]先进纺织材料与制备技术教育部重点实验室,浙江理工大学,杭州310018 [2]合肥工业大学,合肥230009 [3]化学工程联合国家重点实验室聚合反应工程实验室,浙江大学化学与生化工程系,杭州310027

出　　处：《功能高分子学报》2005年第2期325-328,共4页Journal of Functional Polymers

基　　金：浙江省"新世纪151人才工程"基金资助[浙人专(2002)226号]

摘　　要：用凝胶渗透色谱法研究了特种纤维素酶催化水解过程中的分子量及其分布,制备了不同低分子量的壳聚糖和壳寡糖,详细研究了水解温度、pH值、时间和酶的用量等因素对酶催化水解产物的分子量及其分布的影响,制备壳寡糖的反应条件为壳聚糖质量分数w=0.01,水解温度58℃,pH=5.3,特种纤维素酶w=0.10,反应时间3h。Molecular weight and its distribution of chitosan hydrolysis catalyzed process using special celulase was studied by GPC. Thus the different low-molucniar weight chitosan and chitooligosaccharide were prepared. Molecular wight and its distribution of the product of hydrolysis catalyzed are affected by hydrolysis temperature, pH, time and concentration of emzyme. The reaction condition for preparetion chitooligosaccharide is as following: Chitosan concentration of 1%, reaction temperature at 58℃. pH=5.3.10% quaality of emzyme,reaction time for 23h.

关 键 词：特种纤维素酶 催化水解 壳聚糖 壳寡糖 制备方法 水解温度 PH值 时间 反应条件 

分 类 号：O636.1[理学—高分子化学]