CD14在胆道阻塞所致继发性肝损伤中的作用  

Roles of CD14 in the bile duct emphraxis-induced hepatic injury

作　　者：王锋[1] 尹镭[2] 陈玺华[3] 韩德五[1] 

机构地区：[1]山西医科大学病理生理学教研室,太原030001 [2]山西医科大学机能实验室 [3]山西医科大学附属第一临床医学院普外科

出　　处：《山西医科大学学报》2005年第3期284-287,共4页Journal of Shanxi Medical University

摘　　要：目的研究胆总管阻塞小鼠肝脏CD14表达的变化及其影响。方法应用小鼠胆总管阻塞模型,观察胆总管阻塞7,14,28d后血浆内毒素水平(LPS)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、肝组织肿瘤坏死因子(TNF-α)及肝脏CD14表达。结果胆总管阻塞7,14,28d后小鼠出现明显的肝损害,表现为ALT、AST、血浆内毒素(LPS)水平、肝组织TNF-α的增高,肝组织CD14表达水平随着阻塞时间的延长而逐渐增高。结论胆道阻塞后小鼠发生了肠源性内毒素血症(IETM)及肝损害,肝组织CD14表达水平显著升高并发挥了重要的作用。Objective To investigate the role of CD14 in the bile duct emphraxis-induced hepatic injury. Methods A model of bile duct emphraxis was employed. The mice were randomly divided into control and bile duct emphraxis 7,14 and 28 days groups. Results The mice were harvested after bile duct emphraxis on 7,14 and 28 days respectively. Plasma LPS,ALT,AST and liver homogenate TNF-α were observeds. CD14 was observed by immunohistochemistry on frozen. Conclusion Bile duct emphraxis can lead up to IETM. The expression level of CD14 is increased at the same time.

关 键 词：抗原 CD14 内毒素 内毒素血症 

分 类 号：R575[医药卫生—消化系统]