电离辐射诱导PC12细胞分化的蛋白质组学分析  

Proteomic analysis of PC12 cell differentiation induced by ionizing radiation

作　　者：张军权[1] 高荣莲[1] 陈肖华[1] 王治东[1] 董波[1] 饶亚岚[1] 侯丽莉[1] 张浩[1] 毛秉智[1] 

机构地区：[1]放射医学研究所,北京100850

出　　处：《中华放射医学与防护杂志》2005年第3期228-230,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：全军"十五"指令性基金课题(01L018)

摘　　要：目的探索辐射诱导PC12细胞分化的分子机理,筛选神经系统辐射损伤的分子靶标。方法16Gyγ射线照射PC12细胞,照射48h,提取正常与照射后细胞总蛋白,进行双向电泳分析,并对部分差异表达蛋白质进行质谱鉴定。结果电泳中共发现差异表达蛋白质位点876个,鉴定出表达水平增高的蛋白泛素羧端水解酶L1,表达水平降低的蛋白HP1α类似蛋白。结论应用蛋白质组技术鉴定出部分辐射后差异表达蛋白质,为辐射诱导PC12细胞分化的分子机理研究提供了线索。Objective To explore the molecular mechanism of PC12 cell differentiation induced by ionizing radiation and screen the molecular target of nervous system injuried by irradiation. Methods PC12 cells were irradiated with 16 Gy ()^(60)Co γ ray. Total proteins of normal and irradiated cells were prepared 48 hours after irradiation and separated with two dimensional gel electrophoresis. Some differential expressed proteins were characterized with mass spectrometry. Results 876 differential expressed proteins were observed. Up-regulated expression of ubiquitin carboxyl-terminal hydralase L1 was found. Down-regulated expression of new protein similar to HP1α was found. Conclusion The characterization of some differential expressed proteins through proteomic analysis would benefit the research of molecular mechanism of PC12 cell differentiation induced by ionizing radiation.;

关 键 词：细胞分化 辐射诱导 蛋白质组 泛素羧端水解酶 学分 电离 PC12细胞 双向电泳分析 差异表达 Γ射线照射 细胞总蛋白 分子机理 分子靶标 辐射损伤 神经系统 质谱鉴定 技术鉴定 机理研究 水平 

分 类 号：R818[医药卫生—放射医学]