junB启动子X射线辐射敏感区域的检测  

Analysis of the upstream domain of radiation-induced junB gene

作　　者：万虹[1] 石原弘 田中泉 

机构地区：[1]北京市神经外科研究所,100050 [2]日本科学技术厅放射线医学综合研究所

出　　处：《中华放射医学与防护杂志》2005年第3期238-240,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：日本科学技术厅国际交流资助项目

摘　　要：目的探讨junB启动子X射线特异性辐射敏感区。方法构建含250、590、900和1560bpjunB启动子基因片段及CAT报道基因的4种质粒,转染辐射诱导的白血病细胞系L8704细胞,2GyX射线照射,RNA提取,Northern印迹及RNA定量分析。结果含900和1560bpjunB启动子基因片段的质粒组CATRNA表达分别在照射后30和60min达高峰。结论junB启动子590～900bp基因片段为辐射敏感区。Objective To analyze the upstream domain of radiation-induced junB gene. Methods(Four plasmids) containing 250 bp, 590 bp, 900 bp, 1560 bp separately and CAT reporter gene were constructed and transferred to L8704 cells. The cells were incubated for different time intervals after 2 Gy X-irradiation. Total RNA was extracted from the cells and the fluctuation of CAT mRNA level was assessed by the RNA ratio of CAT/β-actin measured by quantitative Northern blot hybridization. Results CAT mRNA expression containing 900 bp and 1560 bp junB promoter was remarkably and rapidly increased, and reached its peak 30 min after 2 Gy X-irradiation. Conclusion The upstream domain of 590-900 bp junB gene is specifically radiosensitive and plays an important role in the early response of the cells to radiation. ;

关 键 词：JUNB 启动子 X射线辐射 敏感区域 NORTHERN印迹 检测 基因片段 白血病细胞系 辐射敏感 X射线照射 RNA提取 RNA表达 报道基因 辐射诱导 定量分析 CAT 特异性 4细胞 质粒 

分 类 号：R818[医药卫生—放射医学]