我国藏族居民乙型肝炎病毒基因型的初步研究  

Preliminary study on genotype of hepatitis B virus detected from Tibetans in China

作　　者：徐烟青[1] 周永东[1] 毕胜利[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所肝炎研究室,北京100052

出　　处：《中华实验和临床病毒学杂志》2005年第2期118-120,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　初步确定流行于西藏和青海藏族居民中乙型肝炎病毒(HBV)基因型的基本情况,为制定肝炎防治策略提供依据。方法　利用PCR扩增西藏和青海藏族居民HBVS基因和C基因测序,并用MEGA3软件进行核苷酸序列的同源性比较及构建系统发生树,分析基因型。结果　绝大多数样本HBV的S基因序列聚集于系统发生树中的基因型D ,所有样本HBV的C基因序列聚集于系统发生树中的基因型C结论　西藏和青海HBV基因型绝大多数为C D重组基因型。Objective To determine the main genotype of hepatitis B virus (HBV) detected from Tibetans in China and provide basic data for hepatitis control and prevention. Methods The S gene and C gene were amplified by PCR from the sera of HBsAg positive Tibetans. After sequencing, the gene sequences were analyzed and the phylogenetic trees were drawn by the software MEGA3. Results In trees based on S gene, the sequences of most samples clustered at genotype D, while in trees based on C gene, the sequences of all samples clustered at genotype C. Conclusion The dominant genotype of HBV detected from Tibetans in China is a C/D hybrid.

关 键 词：乙型肝炎病毒基因型 居民 藏族 乙型肝炎病毒(HBV) 步研究 系统发生树 HBVS基因 HBV基因型 PCR扩增 同源性比较 核苷酸序列 S基因序列 基本情况 防治策略 基因测序 基因型C 青海 西藏 聚集 样本 

分 类 号：R446.5[医药卫生—诊断学]