cyclin E、p16ink4、ki67在宫颈脱落细胞中的表达及其与HPV_(16/18)感染的相关性  

Relations between the expression of cyclin E,p16ink4,ki67 and HPV_(16/18) infection in cervical exfoliated cells

作　　者：赵富玺[1] 郭俊成[1] 崔克[1] 熊思东[2] 

机构地区：[1]山西大同大学医学院微生物教研室,037008 [2]复旦大学上海医学院

出　　处：《中华实验和临床病毒学杂志》2005年第2期138-141,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：山西省科技厅自然基金资助 (2 0 0 5 1115 )

摘　　要：目的　研究cyclinE、p16ink4、ki6 7在宫颈脱落细胞中的表达水平及其与HPV1 6 1 8感染的相关性,探讨其对宫颈癌高危人群筛查的意义。方法　采用免疫组织化学方法对78例宫颈脱落细胞标本进行cyclinE、p16ink4、ki6 7检测,同时应用多重引物PCR技术检测HPV1 6 1 8。结果　cyclinE、p16ink4、ki6 7在宫颈癌细胞中的表达水平均较鳞状上皮非典型增生(ASCUS)差异有统计学意义(P <0 0 0 5 ) ;各级宫颈癌细胞中HPV1 6 的阳性率均较ASCUS差异有统计学意义(χ2 =2 5 2 7,P <0 0 0 5 ) ,且随着宫颈上皮细胞损伤程度加重阳性率升高,差异也有统计学意义(P <0 0 1)。p16ink4和ki6 7在宫颈癌细胞中的表达水平与HPV1 6 高度相关(rs=1 0 ,P <0 0 5 ) ;而cyclinE的表达与HPV1 6 相关性较小(rs=0 4 ,P <0 0 5 )。HPV1 8阳性例数较少,在各项分析中差异均无统计学意义(χ2 =3 6 8,P >0 0 5 )。结论　宫颈癌细胞中cyclinE、p16ink4及ki6 7的高度表达与HPV1 6 感染有关;它们均可能作为有价值的诊断指标应用于宫颈癌高危人群筛查,且cyclinE对宫颈癌的早期诊断意义更大。Objective To confirm the relations between the expression of cyclin E,p16ink4,ki67 and HPV 16/18 infection using cervical exfoliated cells,and evaluate the usefulness of cyclin E,p16ink4 and ki67 as biomarkers for screening of cervical carcinomas.Methods The expression of cyclin E,p16ink4 oncoproteins and ki67 proliferative activity was evaluated immuohistochemically in 78 cervical exfoliated epithelial specimens.Human papillomavirus type16 and 18 (HPV 16/18 ) infection was assessed by polymerase chain reaction(PCR) using type specific primers. Results Cyclin E,p16ink4 and ki67 were all overexpressed in cervical preneoplasia and neoplasia cells, compared with little expressed in ASCUS (P<0.005). Overexpression of cyclin E was observed in CIN Ⅰ (P<0.01), p16ink4 and ki67 overexpressed in invasive carcinoma(100% and 90.9% respectively). The degree of p16ink4 and ki67 expression correlated well with the degree of cervical neoplasia (P<0.005). HPV 16 infection was assessed at all stages of cervical neoplasia samples, and a significant relationship with the degree of cervical epithelial lession was observed at the same time. The expression level of p16ink4 and ki67 seemed to be more closely associated with HPV 16 infection than cyclin E did (r s=1.0 vs r s=0.4). HPV 18 was found positive in only 1 case in CIN Ⅰ and in 4 cases in CIN Ⅱ-Ⅲ -3. Therefore no significance was found on statistical analysis (P<0.005). Conclusion Cyclin E,p16ink4 and ki67 should be regarded as useful biomarkers of HPV-related cervical neoplasias,and be used for screening patients at high risk for developing cervical carcinomas.Moreover, cyclin E might be a significant cytologic marker for the primary screening of cervical carcinomas.

关 键 词：HPV16/18 cyclin 宫颈脱落细胞 KI67 相关性 感染 P16INK4 表达及 宫颈癌细胞 免疫组织化学方法 高危人群筛查 PCR技术检测 脱落细胞标本 宫颈上皮细胞 cvclin 非典型增生 ASCUS 统计学 鳞状上皮 损伤程度 阳性例数 诊断指标 

分 类 号：R737.33[医药卫生—肿瘤] R734.2[医药卫生—临床医学]