氨氧化菌混培物在微量NO_2气氛下的氨代谢特性  

The ammonia metabolic characteristic of mixed ammonia oxidizer culture in the atmosphere of trace NO_2.

作　　者：张代钧[1] 卢培利[1] 李小鹏[1] 祖波[1] 

机构地区：[1]重庆大学环境科学系

出　　处：《中国环境科学》2005年第3期348-352,共5页China Environmental Science

基　　金：国家自然科学基金资助项目(50378094)

摘　　要：用普通活性污泥经120d富集,得到氨氧化菌混培物,氨氧化菌浓度提高300倍.在NO2/O2混合气氛下进行氨氧化试验,结果表明,无分子氧时,氨氧化菌能以NO2为电子受体氧化氨,并产生NO;加入氧气后,氨氧化速率明显提高,最高速率[33(mol NH4+-N/(g MLSS(h)]发生在混合气体中NO2和O2浓度分别为1.79(mol/L和9.38mmol/L时;在NO2浓度为0.89～1.79(mol/L范围内,O2能够强化基于NO2的氨氧化反应,当NO2浓度增大至6.71(mol/L时,氨氧化速率却降低.部分NO被O2氧化,使得反应器出口气体中NO2浓度高于进口.试验过程中产生约20%氮损失,这与氨氧化和厌氧氨氧化相互耦合产生气态含氮产物(N2、NO、N2O)有关.The mixed ammonia oxidizer culture was obtained using common activated sludge enriched for 120 day and the concentration of the ammonia oxidizers increased 300 times. The ammonia oxidation test was made under the mixed atmosphere of NO2/O2. Ammonia oxidizer could use NO2 as electron acceptor to oxidize ammonia and produce NO when there was no molecule oxygen. After adding O2, the ammonia oxidation rate increased obviously. The maximum ammonia oxidation rate 33 μmol NH4+-N/(g MLSS·h) occurred when the concentration of NO2 and O2 in the mixed gas was 1.79 μmol NO2/L and 9.38 mmolO2/L respectively. O2 could strengthen the ammonia oxidation reaction based on NO2, when the concentration of NO2 was in the range of 0.89-1.79 μmol/L; but with increase of NO2 concentration to 6.71 μmol/L the ammonia oxidation rate decreased. Part of NO was oxidized by O2, causing NO2 concentration in gas of reactor outlet higher than that of the inlet. About 20% maximum N loss produced in the test process; this was related with the production of gaseous products containing N (N2, NO and N2O) due to coupling ammonia oxidation and anaerobic ammonia oxidation.

关 键 词：氨氧化菌混培物 微量NO2 氨氧化 厌氧氨氧化 

分 类 号：X703[环境科学与工程—环境工程]