DNA倍体类型与鼻咽癌放射敏感性及预后关系的研究  

Relationship between DNA-ploidy and Radiosensitivity and Prognosis of Nasopharyngeal Carcinoma

作　　者：石小燕[1] 袁响林[1] 陶德定[2] 龚建平[2] 胡国清[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤中心,武汉430030 [2]华中科技大学同济医学院附属同济医院外科/分子医学中心,武汉430030

出　　处：《肿瘤防治研究》2005年第6期368-370,共3页Cancer Research on Prevention and Treatment

基　　金：973肿瘤计划资助项目(G1998051212)

摘　　要：目的探讨DNA倍体类型与鼻咽癌放射敏感性及预后的关系。方法应用流式细胞术(FCM)分析45例放疗前鼻咽癌细胞的DNA倍体,放疗结束六个月评定疗效。对患者进行定期随访,分析DNA倍体性与鼻咽癌放射敏感性及预后有无相关性。结果DNA倍体性与鼻咽癌临床分期、近期疗效、放射敏感性及预后均有明显的相关性,但与性别、病理类型无关。早期肿瘤(Ⅰ+Ⅱ期)的DNA异倍体率为11.11%(2/18)远低于晚期(Ⅲ+Ⅳ期)者的40.74%(11/27)(P<0.05),异倍体肿瘤较二倍体肿瘤对放疗更敏感(P<0.01),且异倍体肿瘤的近期疗效明显好于二倍体肿瘤(P<0.05),但异倍体肿瘤的预后较二倍体肿瘤差(P<0.01)。结论DNA倍体类型可作为评估鼻咽癌放射敏感性和预后的一个独立指标。FCM的肿瘤细胞DNA倍体分析可能成为鼻咽癌选择放疗分割方案的客观指标。Objective To explore the correlation between DNA-ploidy and radiosensitivity and prognosis of nasopharyngeal carcinoma ( NPC ).Methods The DNA-ploidy of 45 cases NPC cells was analyzed before the radiotherapy by means of FCM. The short-term curative effect was assessed in the sixth month after the radiotherapy was end up. The patients were followed up periodically after the treatment. And the relationship between DNA-ploidy and radiosensitivity and prognosis was then statistically analyzed .Results There was no relationship between DNA-ploidy and the sex and pathologic type. But there was a remarkable correlation between DNA-ploidy and the following factors: clinical stage, short-term curative effect, radiosensitivity and prognosis. The percentage of the DNA aneuploidy in early stage (Ⅰ+Ⅱstage) was 11.11% (2/18), while in late stage (Ⅲ+Ⅳstage) it was 40.74% (11/27) ( P < 0.05 ). The aneuploidy tumors were more radiosensitive than the diploidy tumors ( P < 0.01 ) and the short-term curative effect of the aneuploidy tumors was much better than that of the diploidy tumors ( P < 0.05 ). But the prognosis of the aneuploidy tumors were much more poor than that of the diploidy tumors ( P < 0.01 ). Conclusion Based on these findings, DNA-ploidy is considered as an independent factor of radiosensitivity and prognosis for NPC. The analysis of DNA-ploidy by means of FCM may be as an objective index to choose different divide-up radiotherapy for each individual patient of NPC.

关 键 词：流式细胞术 DNA倍体 放射敏感性 预后 鼻咽癌 

分 类 号：R739.63[医药卫生—肿瘤] R730.55[医药卫生—临床医学]