PDGF-B链基因三链形成寡核苷酸对C6胶质瘤细胞增殖和凋亡的影响  

Effects of proliferation and apoptosis of C6 glioma cells with triplex forming oligonucleotides (TFO)

作　　者：李维方[1] 周定标[1] 余新光[1] 金由辛[2] 

机构地区：[1]解放军总医院神经外科,北京100853 [2]中国科学院上海生命科学研究院生物化学与细胞生物学研究所分子生物学国家重点实验室

出　　处：《中华神经外科杂志》2005年第6期323-326,共4页Chinese Journal of Neurosurgery

基　　金：全军"十五"医药卫生科研基金课题。课题编号(01MB066)

摘　　要：目的观察PDGFB链基因三链形成寡核苷酸(triplexformingoligonucleotide,TFO)对C6胶质瘤细胞增殖和凋亡的影响。方法应用流式细胞技术观察TFO对C6胶质瘤细胞PDGFB、PCNA表达的影响,应用流式细胞技术观察TFO对C6胶质瘤细胞凋亡的影响。结果TFO对C6胶质瘤细胞PDGFB、PCNA的表达有明显抑制作用,而且抑制作用存在浓度依赖性。TFO有明显诱导C6胶质瘤细胞细胞凋亡作用,而且诱导作用存在浓度依赖性。结论TFO抑制C6胶质瘤细胞细胞增殖,同时诱导C6胶质瘤细胞细胞凋亡。Objective To observe effects of proliferation and apoptosis of C6 glioma cells by triplex forming oligonucleotide (TFO). Methods The C6 glioma cell expressions of PDGF-B chain and PCNA after adding different dosage of TFO was quantified by immunofluorescence flow cytometric analysis.Apoptosis of C6 glioma cells after adding different dosage of TFO was detected by flow cytometric analysis.Results TFO can obviously inhibit the C6 glioma cell expressions of PDGF-B chain and PCNA in concentration-dependent fashion. TFO can obviously induce the C6 glioma cell apoptosis in concentration-dependent fashion.Conclusions TFO can effectively suppress the C6 glioma cell proliferation and induce the C6 glioma cell apoptosis.

关 键 词：三链形成寡核苷酸 PDGF 细胞增殖 B链基因 C6胶质瘤细胞 流式细胞技术 浓度依赖性 PCNA表达 抑制作用 TFO 瘤细胞凋亡 方法应用 凋亡作用 诱导作用 

分 类 号：R739.4[医药卫生—肿瘤]