神经生长因子对人角膜上皮细胞增殖的影响  

Effect of nerve growth factor on the proliferation of human corneal epithelial cells

作　　者：李倩[1] 邱良秀[2] 李中国[2] 

机构地区：[1]广东省人民医院眼科,广东广州510080 [2]华中科技大学同济医学院附属同济医院眼科,湖北武汉430030

出　　处：《眼视光学杂志》2005年第2期84-87,共4页Chinese Journal of Optometry & Ophthalmology

基　　金：湖北省科技厅资助项目(2001AA301C33)

摘　　要：目的研究神经生长因子(nervegrowthfactor,NGF)在体外培养的人角膜上皮细胞中的表达,观察外源性给予NGF对人角膜上皮细胞增殖的作用,进一步探讨NGF在角膜疾病治疗中的意义。方法采用刮片法进行人角膜上皮细胞培养,外源性给予NGF作用于第2代～第3代培养的人角膜上皮细胞,通过四甲基偶氮唑盐(methylthiazolyltetrazolium,MTT)法测定其对细胞增殖的影响,并用流式细胞仪检测角膜上皮细胞中细胞增殖核抗原的表达情况。结果本实验成功培养出人角膜上皮细胞,加入外源性NGF后,培养的人角膜上皮细胞的增殖率较对照组明显增加(P<0.01),流式细胞仪检测到的细胞增殖核抗原的表达也较对照组明显增多(P<0.01)。结论外源性给予NGF对体外培养的人角膜上皮细胞的细胞增殖有明显的促进作用。Objective To investigate the expression of nerve growth factor (NGF) mRNA in cultured human corneal epithelial cells, and to observe the effect of exogenous NGF on the proliferation of human corneal epithelial cells.Methods Human corneal epithelial cells were cultured primarily in a DMEM medium for seven days. The second and third generations of human corneal epithelial cells were exposed to NGF, and the proliferation characteristics of the cells were measured with a methyl thiazolyl tetrazolium (MTT) assay. The expression of proliferating cell nuclear antigen (PCNA) in the corneal epithelial cells was observed using a flow cytometer (FCM) method.Results Expression of NGF-mRNA can be detected in cultured human epithelial cells. When exogenous NGF was given, the proliferation of human corneal epithelial cells was markedly increased (P<0.01). The FCM test showed that PCNA expression in the cultured cells also increased.Conclusion Exogenous NGF can stimulate the proliferation of cultured human corneal epithelial cells.

关 键 词：神经生长因子 人角膜上皮细胞/生长和发育 细胞增殖 

分 类 号：R772.2[医药卫生—眼科]